FastQCFastQC Report
Sat 1 Jul 2023
SRR2007068.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2007068.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22870593
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG15589436.816364577866434No Hit
TCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTT10576774.6246155488841065TruSeq Adapter, Index 8 (100% over 37bp)
ACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA9694874.239011205350032Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG6773732.9617640434596515TruSeq Adapter, Index 8 (100% over 49bp)
CCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC6347422.7753631049269254TruSeq Adapter, Index 5 (100% over 35bp)
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC4107881.7961405723061052Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC3276881.4327918825716501No Hit
TGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAAC2573691.1253271832523102Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATAGATCGGAAGAGCACACG2046060.8946248136198305No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC1962470.8580756957198267No Hit
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC1721460.7526958308426895Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA1640190.7171611160235329No Hit
TCTCGGCGCCCCCTCGATGCTCTAGATCGGAAGAGCACACGTCTGAACTC1553700.6793439942724703Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATATTAAATGGATTTTTGGAAGTAGGAGTTGGAGATCGGAAGAGCACACG1364730.596718239881231No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTC1202690.5258674315965485TruSeq Adapter, Index 8 (100% over 50bp)
CGGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT1197410.5235587901022067TruSeq Adapter, Index 8 (100% over 43bp)
TCCGCGTCGGTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTT1118580.48909094748876863TruSeq Adapter, Index 8 (100% over 37bp)
TCCCGTGGATCGCCTCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGT1052620.4602504185177883Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACCTCCGCGTCGGTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA1043400.45621904075683567Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACA997480.4361408556393794No Hit
CTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACT916070.40054492684120613TruSeq Adapter, Index 8 (100% over 36bp)
ACCTCCGCGTCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACT779940.3410230771016738TruSeq Adapter, Index 8 (100% over 36bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA767130.3354219980216516No Hit
TATATTAAATGGATTTTTGGAAGTAGGAGTTGGAGATCGGAAGAGCACAC738740.3230086775624926No Hit
CCTCCGCGTCGGTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC676620.29584716058739713TruSeq Adapter, Index 5 (100% over 35bp)
TCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCCA657930.28767509438867633Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAGATCGGAAGAGCACACG638310.2790963924722022No Hit
GTTTCTGTAGTGTAGTGGTTATCACGCTCGCCTGAGATCGGAAGAGCACA624800.2731892434970969No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC569450.24898785965016298No Hit
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACAC541750.2368762366590145No Hit
ATATATTAAATGGATTTTTGGAAGTAGGAGTTGGAGATCGGAAGAGCACA536840.23472937496635962No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA523620.22894902637636025No Hit
TCCGCGTCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTG519960.2273487180677825TruSeq Adapter, Index 8 (100% over 38bp)
CGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT509010.2225609104232671TruSeq Adapter, Index 8 (100% over 43bp)
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC504990.22080319474007518TruSeq Adapter, Index 5 (100% over 35bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA504870.22075072561520376No Hit
TGGGGGGAACCTCCGCGTCGGTAGATCGGAAGAGCACACGTCTGAACTCC495020.21644388494867625Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACACG482650.21103519265984927No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC480550.21011698297459974No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACAC450710.19706966058991127No Hit
TCTCGGCGCCCCCTCGATGCTCCAGATCGGAAGAGCACACGTCTGAACTC449390.19649250021632586Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGGCGCCCCCTCGATGCTCTAGATCGGAAGAGCACACGTCTGAACTCCA435790.190545999397567Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACT433650.189610300004027Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG431510.18867460061048702Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACAGATCGGAAGAGCAC430430.1882023784866444No Hit
AACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC423520.1851810313794662Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGGGGGGAACCTCCGCGTCGGTAAGATCGGAAGAGCACACGTCTGAACTC419580.18345829511285516Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTTAGATCGGAAGAGCACA410360.17942691735190253No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG409370.17899404707171343No Hit
CCTCCGCGTCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACT384390.1680717242443167TruSeq Adapter, Index 8 (100% over 36bp)
TTGGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATC357870.15647604764773698TruSeq Adapter, Index 8 (100% over 42bp)
GTTTCCGTAGTGTAGTGGTCATCACGCTCGCCTGAGATCGGAAGAGCACA355610.1554878791293256No Hit
TCTCGGCGCCCCCTCGATGCTTAGATCGGAAGAGCACACGTCTGAACTCC355490.15543541000445418Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT354840.1551512022447341No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG350320.1531748652079113No Hit
ACCTCCGCGTCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC348590.15241843532434862TruSeq Adapter, Index 5 (100% over 35bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC327920.1433806285652497No Hit
TCCGGAGCGGGAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC313820.13721550639286004TruSeq Adapter, Index 5 (100% over 35bp)
TCCGCGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTT303770.13282121718488019TruSeq Adapter, Index 8 (100% over 37bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTAGATCGGAAGAGCACAC299600.13099791509559897No Hit
TCCGGAGCGGGAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA297470.13006658812913158Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACCTCCGCGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA293000.12811211322767188Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATGGTTAGCACTCTGGACTCAGATCGGAAGAGCACACGTCTGAACTCCAG287760.1258209614416207Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA280360.12258536540788426No Hit
TCCGCGTCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGA264090.11547142656073675TruSeq Adapter, Index 8 (100% over 39bp)
TCCCGTGGATCGCCTCAGTAGATCGGAAGAGCACACGTCTGAACTCCAGT263380.11516098423858095Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGTGGGGGGAACCTCCGCGTCGGTAAGATCGGAAGAGCACACGTCTGAAC261510.11434334037600162Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAGATCGGAAGAGCACA260570.11393233223117563No Hit
GTTTCCGTAGTGTAGTGGTCATCACGCTCGCCTAGATCGGAAGAGCACAC250960.10973042981439089No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC232780.10178135739637359No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)