FastQCFastQC Report
Fri 30 Jun 2023
SRR2007069.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2007069.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21153634
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG9527444.503925897555002No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC3762751.7787723849245005No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA3762091.7784603817953926No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC2715611.2837557839943718No Hit
ACGTTGAACGCACGTTCGTGTGGAACCTGGCGCTAGATCGGAAGAGCACA2560421.2103925027728095No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC1994400.9428167283219516No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA1839340.8695149022621834No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG1436990.6793111765099084TruSeq Adapter, Index 4 (100% over 49bp)
ACGTTGAACGCACGTTCGTGTGGAACCTGGCGCTAAACCAGATCGGAAGA1330670.6290503088027334No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAGATCGGAAGAGCACA1087120.5139164268418371No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCAC1057820.5000653788375085No Hit
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC942050.4453371935999271Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAAC937570.4432193541781048Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGAGGCCCTGTAATTGGAATGAGTCCACTTTAGATCGGAAGAGCACACG925720.4376174798145793No Hit
ACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT789040.373004468168448Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACAC760290.3594134227717091No Hit
TCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCAC751770.3553857460141364No Hit
ATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCAGATCGGAAGAGCA738530.3491267741514295No Hit
TTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAGATCGGAAGAGCACAC681780.3222992323683014No Hit
ATATTAAATGGATTTTTGGAAGTAGGAGTTGGAGATCGGAAGAGCACACG678370.3206872162012447No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT673720.3184890123370765No Hit
AATCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTC554650.26220081145395635No Hit
TCTCGGCGCCCCCTCGATGCTCTAGATCGGAAGAGCACACGTCTGAACTC549820.25991751582730416Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACA547160.2586600486705972No Hit
ACGTTGAACGCACGTTCGTGTGGAACCTGGCGCAGATCGGAAGAGCACAC525320.24833558148921364No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG506860.23960894851447276No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACTAGATCGGAAGAGCACAC491460.23232887550195866No Hit
GTTTCTGTAGTGTAGTGGTTATCACGCTCGCCTGAGATCGGAAGAGCACA482510.2280979239784521No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCA470730.22252914085589265No Hit
TGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGC466940.22073748652359212No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA457720.21637889735635968No Hit
ATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTGAACT456220.21566979933566027Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA438540.20731189733168304No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACAC425030.20092528782525024No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGGAAGAGCAC393240.18589713710656053No Hit
GTTTCCGTAGTGTAGTGGTCATCACGCTCGCCTGAGATCGGAAGAGCACA378280.1788250661801183No Hit
TATATTAAATGGATTTTTGGAAGTAGGAGTTGGAGATCGGAAGAGCACAC374780.17717050413181962No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGATCGGAAGAGCA357440.1689733310125343No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACACG346690.16389146186418846No Hit
GTTGAACGCACGTTCGTGTGGAACCTGGCGCTAAACCAGATCGGAAGAGC345710.16342818449066482No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTC323620.1529855343058313TruSeq Adapter, Index 4 (100% over 50bp)
AAATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGACTAGATCGGAAGAG320570.1515437016637425No Hit
ACGTTGAACGCACGTTCGTGTGGAACCTGGCGCCAGATCGGAAGAGCACA318630.15062660155697125No Hit
ATCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTCT303580.14351198474928706Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATATTAAATGGATTTTTGGAACTAGGAGTTGGAGATCGGAAGAGCACACG301600.1425759753619638No Hit
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC300130.14188105930167838Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC295540.13971121935833813No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGAGATCGGAAGAGCACACGT294940.13942758015005838No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACAC291940.13800938410865954No Hit
AGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAGATCGGAAGAGC290950.13754137941499792No Hit
CCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG279160.13196786897230045TruSeq Adapter, Index 4 (100% over 35bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACAGATCGGAAGAGCAC277600.13123040703077307No Hit
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACAC276830.13086640338014735No Hit
TCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC274360.1296987553060623TruSeq Adapter, Index 4 (100% over 37bp)
ACGGACCAAGGAGTCTAACGCGTGCGCGAGTCAGATCGGAAGAGCACACG262820.12424342786681475No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACAC259820.12282523182541591No Hit
ATATATTAAATGGATTTTTGGAAGTAGGAGTTGGAGATCGGAAGAGCACA242280.11453351230337067No Hit
TGGGGCGAAGCTACCATCTGTGGGATTATGACTGAGATCGGAAGAGCACA239950.11343204671121757No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCAGATCGGAAGAGCAC226760.10719671144920065No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAGATCGGAAGAGCACAC226030.10685161707912692No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATAGATCGGAAGAGCACACG221370.10464868589482072No Hit
ATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAGATCGGAAGAGCA217840.1029799418861081No Hit
TGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAGATCGGAAGAGC215960.1020912057001648No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC212930.10065882769835198No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA212360.10038937045048618No Hit
GTTGAACGCACGTTCGTGTGGAACCTGGCGCTAGATCGGAAGAGCACACG211900.10017191372413838No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)