FastQCFastQC Report
Fri 30 Jun 2023
SRR2007070.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2007070.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23215960
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG16493727.104474680349209No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG7937773.4191004808760868TruSeq Adapter, Index 9 (100% over 49bp)
TCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATC6867512.9580986528233164TruSeq Adapter, Index 9 (100% over 37bp)
ACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG6587602.8375307331680446Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA4808082.0710235544857936TruSeq Adapter, Index 9 (100% over 35bp)
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC4377191.8854227867380886Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAAC3853701.6599356649477341Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC2350171.0123079123154932No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATAGATCGGAAGAGCACACG1968500.8479080770297674No Hit
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC1652760.7119068089366108Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCT1452700.6257333317252441TruSeq Adapter, Index 9 (100% over 43bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC1420550.6118850997331146No Hit
TCTCGGCGCCCCCTCGATGCTCTAGATCGGAAGAGCACACGTCTGAACTC1414140.6091240680979808Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCGTGGATCGCCTCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGT1356110.5841283324058105Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATATTAAATGGATTTTTGGAAGTAGGAGTTGGAGATCGGAAGAGCACACG1258440.5420581358686007No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA1152320.49634820184045797No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTC1031490.4443021094109397TruSeq Adapter, Index 9 (100% over 50bp)
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACA818810.352692716562227No Hit
TATATTAAATGGATTTTTGGAAGTAGGAGTTGGAGATCGGAAGAGCACAC704540.30347226649253356No Hit
TCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCCA697410.3004011033788825Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCTCCGCGTCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAT678650.29232045541084667TruSeq Adapter, Index 9 (100% over 36bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAGATCGGAAGAGCACACG676680.29147190122656996No Hit
CTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAT600690.2587401080980498TruSeq Adapter, Index 9 (100% over 36bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA592660.25528128063625194No Hit
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACAC576030.24811810495883002No Hit
TCCGCGTCGGTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATC572810.24673112806879405TruSeq Adapter, Index 9 (100% over 37bp)
ACCTCCGCGTCGGTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG560480.24142012649918418Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCT559860.24115306883712756TruSeq Adapter, Index 9 (100% over 43bp)
ATATATTAAATGGATTTTTGGAAGTAGGAGTTGGAGATCGGAAGAGCACA549300.23660447381887287No Hit
TGGGGGGAACCTCCGCGTCGGTAGATCGGAAGAGCACACGTCTGAACTCC535900.23083258241313304Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACACG524340.22585324923027092No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA508360.21897005335984382No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC504960.21750554360017849No Hit
GTTTCTGTAGTGTAGTGGTTATCACGCTCGCCTGAGATCGGAAGAGCACA503790.21700157994758779No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC463750.19975482383670545No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG456560.19665781643317787No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACAC418120.18010024138566746No Hit
CCTCCGCGTCGGTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA403200.17367362796972427TruSeq Adapter, Index 9 (100% over 35bp)
GTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACT401050.17274754091581826Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTCGGCGCCCCCTCGATGCTCCAGATCGGAAGAGCACACGTCTGAACTC373400.16083763066442222Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATC362770.15625888397464502TruSeq Adapter, Index 9 (100% over 42bp)
TCGCGTCGGGGTCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC356900.1537304509483993Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGGGGGGAACCTCCGCGTCGGTAAGATCGGAAGAGCACACGTCTGAACTC352470.15182228087918828Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA342680.14760535424768134No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG335200.1443834327764176Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCGGAGCGGGAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA333030.14344873095921942TruSeq Adapter, Index 9 (100% over 35bp)
AACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC327740.141170126068446Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT326550.14065754765256314No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC316780.13644923578434834No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG313450.13501487769620554No Hit
TCGGCGCCCCCTCGATGCTCTAGATCGGAAGAGCACACGTCTGAACTCCA313400.13499334078797517Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTGGGGGGAACCTCCGCGTCGGTAAGATCGGAAGAGCACACGTCTGAAC309930.1334986793567873Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCGTGGATCGCCTCAGTAGATCGGAAGAGCACACGTCTGAACTCCAGT298960.12877348169104358Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTAGATCGGAAGAGCACAC296390.12766648460800242No Hit
ACGTTGAACGCACGTTCGTGTGGAACCTGGCGCTAGATCGGAAGAGCACA294740.12695576663640012No Hit
TCCGCGTCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCA293570.1264518029838094TruSeq Adapter, Index 9 (100% over 38bp)
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA290070.12494421940768333TruSeq Adapter, Index 9 (100% over 35bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACAGATCGGAAGAGCAC289460.12468146912727278No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA284830.1226871514251403No Hit
ACGGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATC283860.12226933540547107TruSeq Adapter, Index 9 (100% over 42bp)
TCTCGGCGCCCCCTCGATGCTTAGATCGGAAGAGCACACGTCTGAACTCC282250.12157584696045308Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCGGAGCGGGAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG274920.11841853621388046Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTTTCCGTAGTGTAGTGGTCATCACGCTCGCCTGAGATCGGAAGAGCACA270540.11653190305289982No Hit
CGCGTCGGGGTCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA266240.11467972894508777Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCTCCGCGTCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAT263640.1135598097171084TruSeq Adapter, Index 9 (100% over 36bp)
ATATATTAAATGGATTTTTGGAAGTAGGAGTAGATCGGAAGAGCACACGT261660.11270694815118565No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTTAGATCGGAAGAGCACA259580.11181101276880215No Hit
ATGGTTAGCACTCTGGACTCAGATCGGAAGAGCACACGTCTGAACTCCAG253590.10923089116280352Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACACGTCTGAACTCCA243800.10501396453129656Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)