FastQCFastQC Report
Fri 30 Jun 2023
SRR2007071.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2007071.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22260855
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG6257872.811154378392025No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC5953992.67464569532482No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC4067671.8272748283927098No Hit
ACGTTGAACGCACGTTCGTGTGGAACCTGGCGCTAGATCGGAAGAGCACA3979301.7875773414812686No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA3037631.364561244390658No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA2743111.232257251574569No Hit
TCGAGGCCCTGTAATTGGAATGAGTCCACTTTAGATCGGAAGAGCACACG1501950.674704543019574No Hit
TCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCAC1464490.6578767976342328No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC1459540.6556531633668159No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACAC1176020.5282905800338756No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATAGATCGGAAGAGCACACG1174440.5275808139444779No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACAGATCGGAAGAGCAC1099280.49381751060325396No Hit
TTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAGATCGGAAGAGCACAC1063050.4775423046419376No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAGATCGGAAGAGCACA1037890.46623995349684455No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG991040.44519404128907TruSeq Adapter, Index 5 (100% over 49bp)
ACGTTGAACGCACGTTCGTGTGGAACCTGGCGCCAGATCGGAAGAGCACA987440.44357685273094855No Hit
TGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGC865700.3888889263238092No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCAC793850.3566125380179692No Hit
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACA780800.3507502294947791No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACACG769580.3457099918219673No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACTAGATCGGAAGAGCACAC749800.33682443913317794No Hit
TCTCGGCGCCCCCTCGATGCTCTAGATCGGAAGAGCACACGTCTGAACTC711940.31981700613026764Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTCAGATCGGAAGAGC680210.3055632858666031No Hit
TGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAAC651200.29253144140240794Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC603220.2709779116750008Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCAGATCGGAAGAGCA529220.2377357024247272No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC523060.23496851311416386No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAGATCGGAAGAGCAC516650.23208901904261986No Hit
AATCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTC511850.2299327676317913No Hit
AGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGATCGGAAGAGCA499060.22418725606002105No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACAC484480.21763764239962932No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG461110.20713939334315776No Hit
ATATTAAATGGATTTTTGGAAGTAGGAGTTGGAGATCGGAAGAGCACACG434010.19496555725285483No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA416910.1872839116017781No Hit
ACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA414460.1861833249441677Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACAC403560.18128683736541115No Hit
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACAC392740.17642628731016846No Hit
TCTCCTACTTGGATAACTGTGGTAATTCTAGAGCCAGATCGGAAGAGCAC389610.17502023170269065No Hit
ACGTTGAACGCACGTTCGTGTGGAACCTGGCGCAGATCGGAAGAGCACAC387380.17401847323474323No Hit
ATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTGAACT382000.17160167477843954Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGTTGAACGCACGTTCGTGTGGAACCTGGCGCTAAACCAGATCGGAAGA381090.17119288544846997No Hit
GGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCA370260.16632784320278804No Hit
GTTGAACGCACGTTCGTGTGGAACCTGGCGCTAGATCGGAAGAGCACACG369580.16602237425292066No Hit
AAATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGACTAGATCGGAAGAG366290.16454444359841525No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA354460.1592301823088107No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAGATCGGAAGAGCACAC337920.15180009932233063No Hit
TTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAGATCGGAAGAGCACA334560.15029072333475063No Hit
ATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCAGATCGGAAGAGCA328890.14774365135570938No Hit
GGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGC328880.14773915916527017No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCCAGATCGGAAGAGCACAC319200.1433907188200992No Hit
GTTTCTGTAGTGTAGTGGTTATCACGCTCGCCTGAGATCGGAAGAGCACA318050.14287411691958823No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCA312840.1405336857007514No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGT304290.136692862875213No Hit
ATCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTCT297650.13371004842356685Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAAGACGGTCGAACTTGACTATCTAGAGGAAGTAGATCGGAAGAGCACA296600.1332383684274481No Hit
TATATTAAATGGATTTTTGGAAGTAGGAGTTGGAGATCGGAAGAGCACAC277790.12478855821126368No Hit
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC277310.12457293307018083Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAGATCGGAAGAGC276600.12425398754899576No Hit
GTTTCCGTAGTGTAGTGGTCATCACGCTCGCCTGAGATCGGAAGAGCACA266760.11983367215679722No Hit
TGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAG264380.11876453083226139No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT261910.11765495979377252No Hit
TCGAGGCCCTGTAATTGGAATGAGTCCACTTCAGATCGGAAGAGCACACG256850.11538191143152408No Hit
GTTGATTAAGTCCCTGCCCTTTGTAGATCGGAAGAGCACACGTCTGAACT253830.11402526991887779Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGTTGAACGCACGTTCGTGTGGAACCTGGCGCAAGATCGGAAGAGCACA247430.11115026803777305No Hit
GTTGAACGCACGTTCGTGTGGAACCTGGCGCTAAACCAGATCGGAAGAGC247170.11103347108635316No Hit
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAG246720.11083132251658798No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTAGATCGGAAGAGCACACG242950.1091377667209997No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)