FastQCFastQC Report
Fri 30 Jun 2023
SRR2007072.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2007072.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23138057
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG16476837.121094913025757No Hit
TCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGC14989216.478162794741149TruSeq Adapter, Index 10 (100% over 37bp)
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC7457803.2231747030444255Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAAC4791152.070679487045952Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT4100011.7719767913096591Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG3480751.5043397982812472TruSeq Adapter, Index 10 (100% over 49bp)
CCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA2818071.217937184613211TruSeq Adapter, Index 10 (100% over 35bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA1899350.8208770511715828No Hit
TCCCGTGGATCGCCTCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGT1854450.8014717916893368Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC1749230.7559969274861758No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC1515160.6548345870182617No Hit
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC1254750.5422884039053063Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCCA1037400.4483522536053913Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATATTAAATGGATTTTTGGAAGTAGGAGTTGGAGATCGGAAGAGCACACG1024900.4429498985156792No Hit
TCTCGGCGCCCCCTCGATGCTCTAGATCGGAAGAGCACACGTCTGAACTC905930.3915324437138348Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA857880.3707657907489812No Hit
GTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACT837770.3620744818806523Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAG662000.2861087255511558TruSeq Adapter, Index 10 (100% over 36bp)
TATATTAAATGGATTTTTGGAAGTAGGAGTTGGAGATCGGAAGAGCACAC646560.2794357365443434No Hit
GTTTCTGTAGTGTAGTGGTTATCACGCTCGCCTGAGATCGGAAGAGCACA584680.2526919179082323No Hit
CGGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCT580740.25098909558395505TruSeq Adapter, Index 10 (100% over 43bp)
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC575710.24881518789585488No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA572260.2473241378910943No Hit
TCCGCGTCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTT537960.23250007552492413TruSeq Adapter, Index 10 (100% over 39bp)
ACCTCCGCGTCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAG532860.2302959146483216TruSeq Adapter, Index 10 (100% over 36bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATAGATCGGAAGAGCACACG524750.22679086666611636No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAGATCGGAAGAGCACACG519500.22452187752843725No Hit
TGGGGGGAACCTCCGCGTCGGTAGATCGGAAGAGCACACGTCTGAACTCC512380.22144469606933717Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTC507950.21953010142554322TruSeq Adapter, Index 10 (100% over 50bp)
ATATATTAAATGGATTTTTGGAAGTAGGAGTTGGAGATCGGAAGAGCACA499460.21586082184861072No Hit
TCCGCGTCGGTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGC475970.20570871616402364TruSeq Adapter, Index 10 (100% over 37bp)
TCGCTGCGATCTATTGAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT445760.1926523043832073Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG421320.18208961971180207No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACACG415300.1794878455005967No Hit
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACA409520.1769897965071138No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC400280.17299637562479858No Hit
TGGGGGGAACCTCCGCGTCGGTGTAGATCGGAAGAGCACACGTCTGAACT390940.16895973590176563Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC379960.1642143071909625No Hit
TCGCGTCGGGGTCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC378910.16376050936342668Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCGCGTCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCT378540.16360059965277118TruSeq Adapter, Index 10 (100% over 38bp)
TCCGGAGCGGGAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA360860.1559595086138823TruSeq Adapter, Index 10 (100% over 35bp)
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACAC347680.15026326540728982No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACAC342990.1482363017776298No Hit
TGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACACGTCTGAACTCCA341410.1475534440942902Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT332560.143728576690774No Hit
TCGCTGCGATCTATTGAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC322240.13926839232870764Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCGGAGCGGGAGGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCA311220.1345056760816174Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA295490.12770735243672363No Hit
CCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCT295310.12762955852343175TruSeq Adapter, Index 10 (100% over 38bp)
TGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGC284810.12309158024807354No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC284550.12297921126220755No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG284330.12288412981262861No Hit
ATGGTTAGCACTCTGGACTCAGATCGGAAGAGCACACGTCTGAACTCCAG281800.12179069314247086Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGTCGGGGTCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA277560.1199582142960405Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCGGAGCGGGAGGAACCAGATCGGAAGAGCACACGTCTGAACTCCAGTC276380.11944823197557167Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCGGAGCGGGAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT264990.11452560601782596Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTTTCCGTAGTGTAGTGGTCATCACGCTCGCCTGAGATCGGAAGAGCACA258470.1117077376030321No Hit
AACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC252060.10893740991302771Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA237790.1027700813426123No Hit
TGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGT235710.1018711294556842No Hit
TGGGGGGAACCTCCGCGTCGGTAAGATCGGAAGAGCACACGTCTGAACTC231950.10024610104469878Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)