FastQCFastQC Report
Fri 30 Jun 2023
SRR2007073.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2007073.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22787710
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC8520883.739243653706318No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA5184212.2750026220274No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA4032471.7695810592639627No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG3128471.3728759932437267No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC2406371.0559946567689338No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACAGATCGGAAGAGCAC1705490.7484253573527133No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG1564790.6866815489577496TruSeq Adapter, Index 1 (100% over 49bp)
TGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAAC1124140.4933097709247661Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC1082830.47518157814014655Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACACG932040.40900994439546584No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCAGATCGGAAGAGCA733450.3218620914519274No Hit
ATATTAAATGGATTTTTGGAAGTAGGAGTTGGAGATCGGAAGAGCACACG684030.3001749627321043No Hit
ACGTTGAACGCACGTTCGTGTGGAACCTGGCGCTAGATCGGAAGAGCACA664770.29172303842729264No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA543970.23871200748122562No Hit
TGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGC533570.2341481438898424No Hit
CCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCA526070.23085689610759486No Hit
AATCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTC522230.2291717772430841No Hit
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACA493300.21647633746436126No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG483780.21229864694609504No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGAGATCGGAAGAGCACACGT460330.2020080122136011No Hit
TCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA433850.19038771337707913TruSeq Adapter, Index 1 (100% over 37bp)
TATATTAAATGGATTTTTGGAAGTAGGAGTTGGAGATCGGAAGAGCACAC399700.17540156514191202No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTCAGATCGGAAGAGC392580.17227707391396502No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACAC389120.17075871160375483No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC360330.15812470845030063No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACAC344720.1512745247328494No Hit
TCTCGGCGCCCCCTCGATGCTCTAGATCGGAAGAGCACACGTCTGAACTC338560.14857131322103012Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCAC335920.14741279400167898No Hit
CCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGC329820.14473591247211764No Hit
ATATTAAATGGATTTTTGGAAGTAGGAGTAGATCGGAAGAGCACACGTCT313550.13759609894982866Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATAGATCGGAAGAGCACACG312540.13715287758181932No Hit
TGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC280050.12289519218912301No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGATCGGAAGAG278210.12208773939987826No Hit
TGGGGGGAACCTCCGCGTCGGTGTAGATCGGAAGAGCACACGTCTGAACT262100.11501813916361056Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGAACTGGTGCGGACCAGGGGAATCCGACTGTAGATCGGAAGAGCACACG260030.11410975477571025No Hit
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACAC248420.10901490320879105No Hit
ACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA245460.10771595741739737Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)