FastQCFastQC Report
Fri 30 Jun 2023
SRR2007075.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2007075.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20132638
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCGTGGATCGCCTCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGT9091204.5156526432353274Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC8667794.305342399739169No Hit
TCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCCA7903333.925630610355185Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA6366413.1622333844178794No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC5603482.7832815550550305No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA5198672.582210041227583No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA3826591.900689815214479No Hit
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC2586851.2849036475001439Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACTAGATCGGAAGAGCA2188931.087254437297288No Hit
TCCCGTGGATCGCCTCAGTAGATCGGAAGAGCACACGTCTGAACTCCAGT2023201.0049353691254967Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG1932630.9599487161096326TruSeq Adapter, Index 7 (100% over 49bp)
TGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAAC1890580.9390622331758015Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC1601100.7952758103533177No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC1472650.7314739379906399No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGAGATCGGAAGAGCACACGTCT1270180.6309058951936651Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC1261900.6267931703734007Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACATAGATCGGAAGAGCACA1232760.6123191605590882No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA1106140.5494262599863962No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT1022410.5078370753003159No Hit
TCCCGTGGATCGCCTCAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG1015650.5044793434422254Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCGTGGATCGCCTCAGCTAAGATCGGAAGAGCACACGTCTGAACTCCA968050.4808361427846664Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAATAGATCGGAAGAGCAC810910.40278377826095124No Hit
TGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACACGTCTGAACTCCA794890.3948265498043525Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGGGGGAACCTCCGCGTCGGTAGATCGGAAGAGCACACGTCTGAACTCC791690.39323709093661746Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC763560.3792647540774339Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA755610.37531594220290454No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACACG745940.37051279618696764No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAAGATCGGAAGAGCA677050.3362947270000086No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGAGATCGGAAGAGCACACG613860.30490788142120273No Hit
TTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCC599760.2979043282852451Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCCCGTGGATCGCCTCAGCAGATCGGAAGAGCACACGTCTGAACTCCAG578420.2873046244610369Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGCGTGCATTTATCAGATCAAAACCAACCCGGTAGATCGGAAGAGCACAC565980.2811256031127168No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA533680.26508200266651594No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG506140.251402722286071No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC488480.24263089615975808No Hit
TCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA487240.24201498084851078TruSeq Adapter, Index 7 (100% over 37bp)
TTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCAGATCGGAAGAGC470100.2335014417882048No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTAAGATCGGAAGAGCACA441350.21922114727339756No Hit
CGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACACG431200.21417958242730037No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGAGATCGGAAGAGCA403860.2005996432260889No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC382130.18980622410237546No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAAGATCGGAAGAGCACAC377820.1876654216898948No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCAC368400.1829864521479997No Hit
TCCCGTGGATCGCCTCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGT363950.1807761109100556Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACAGATCGGAAGAGCACACG358480.17805912965802098No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCAGATCGGAA337440.16760843760266292No Hit
TGGGGGGAACCTCCGCGTCGGTAAGATCGGAAGAGCACACGTCTGAACTC328900.16336656924939494Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGCGTGCATTTATCAGATCAAAACCAACCCGAGATCGGAAGAGCACACGT322270.16007340915780635No Hit
TCCCGTGGATCGCCTCAGCTTAGATCGGAAGAGCACACGTCTGAACTCCA284760.14144197099257436Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCAGATCGGAAG276180.1371802344034597No Hit
TTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCAAGATCGGAAGAG272160.13518347670086753No Hit
TCTCGGCGCCCCCTCGATGCTTAGATCGGAAGAGCACACGTCTGAACTCC270850.13453279197688847Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCAC264110.13118499423672147No Hit
CGGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCT259950.12911869770866588TruSeq Adapter, Index 7 (100% over 43bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC257660.127981241206443No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA252780.12555731643314702No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTC243900.1211465680751822TruSeq Adapter, Index 7 (100% over 50bp)
TGTGGGGGGAACCTCCGCGTCGGTAAGATCGGAAGAGCACACGTCTGAAC242790.1205952245304366Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAACATTAGACTGTGAATCTGACAACAGGAGATCGGAAGAGCACACGTCT239360.11889152330658308Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGCCCGTCACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTCTGA237900.11816633269817894Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCA227600.1130502619676567No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCTAGATCGGAA224810.11166445251735019No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACAAGATCGGAAGAGCACAC223390.11095913014479275No Hit
TCGCTGTGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC216350.1074623206357756No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAG213600.10609637942131578No Hit
TCCCGTGGATCGCCTCAGCCAGATCGGAAGAGCACACGTCTGAACTCCAG208560.10359298170463305Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCAGATCGGAAGAGCA208500.10356317935086301No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)