FastQCFastQC Report
Fri 30 Jun 2023
SRR2007077.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2007077.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22615405
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC10105484.468405496165114No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG8615213.809443164957691TruSeq Adapter, Index 8 (100% over 49bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA5181222.291013581229255No Hit
TGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAAC3841521.6986297614391606Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC2994841.3242477859671318No Hit
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC2844811.257908049844785Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA2710101.1983424572763566No Hit
TCCCGTGGATCGCCTCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGT2646601.1702642512924264Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTT2519941.1142581793251105TruSeq Adapter, Index 8 (100% over 37bp)
ACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA2086210.9224729780430639Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG1860260.822563204152214No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA1675210.7407384479738479No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC1551660.6861075448350361No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC1448710.6405854770232945No Hit
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC1438040.63586745406505Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGCCCGTCACGTTGAACGCACGTTCGTGAGATCGGAAGAGCACACGTCT1386110.6129052298643336Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA1339160.592145044495113No Hit
TCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCCA1248170.5519114072907384Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT1157060.5116247089097012TruSeq Adapter, Index 8 (100% over 43bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACATAGATCGGAAGAGCACA1076900.4761798428991212No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTC853430.3773666666592971TruSeq Adapter, Index 8 (100% over 50bp)
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACTAGATCGGAAGAGCA834610.3690449054527213No Hit
TCGCCCGTCACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTCTGA746100.3299078658993726Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA669500.29603714812978144No Hit
TGGGGGGAACCTCCGCGTCGGTAGATCGGAAGAGCACACGTCTGAACTCC666060.2945160610654552Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC665310.2941844287113143No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGAGATCGGAAGAGCA627650.27753206276871895No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA596630.2638157486014511No Hit
CCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC574590.25407017915443036TruSeq Adapter, Index 5 (100% over 35bp)
TGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACACGTCTGAACTCCA559320.2473181444241215Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACAGATCGGAAGAGCACACG547760.24220658440562973No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA545980.24141951028513528No Hit
TCCCGTGGATCGCCTCAGTAGATCGGAAGAGCACACGTCTGAACTCCAGT528050.2334912861388067Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTGGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATC516920.22856986200335566TruSeq Adapter, Index 8 (100% over 42bp)
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAGATCGGAAGAGCACAC504570.22310898257183542No Hit
TCTCGGCGCCCCCTCGATGCTCTAGATCGGAAGAGCACACGTCTGAACTC487350.21549470372076024Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCCACCTATAACTTCTCTGTTAACCCAACACCGGAGATCGGAAGAGCAC446780.1975556042440982No Hit
GTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACT444560.19657397247584116Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT442170.19551717070731214TruSeq Adapter, Index 8 (100% over 43bp)
TGTGGGGGGAACCTCCGCGTCGGTAAGATCGGAAGAGCACACGTCTGAAC437680.19353179834718856Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGCCCGTCACGTTGAACGCACGTTCAGATCGGAAGAGCACACGTCTGAA405110.17913011064802953Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGC379160.16765563119475418No Hit
TCCGGAGCGGGAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC354610.1568001988025419TruSeq Adapter, Index 5 (100% over 35bp)
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACAC338410.14963693995309835No Hit
TGGGGGGAACCTCCGCGTCGGTAAGATCGGAAGAGCACACGTCTGAACTC327710.1449056517006881Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCAC312730.13828184814731376No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGAGATCGGAAGAGCACACG300030.13266620695052775No Hit
TCCGCGTCGGTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTT298620.13204273812474285TruSeq Adapter, Index 8 (100% over 37bp)
TCGCTGTGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC298050.13179069753559577No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCAGATCGGAAG295900.13084001812039184No Hit
TGTGGGGGGAACCTCCGCGTCGGTAGATCGGAAGAGCACACGTCTGAACT293710.12987165164630038Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA277510.12270839279685684No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACGAGATCGGAAGAGCAC268430.11869343042939094No Hit
TGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG268080.11853866866412517TruSeq Adapter, Index 8 (100% over 49bp)
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAAGATCGGAAGAGCACAC266800.11797268277972471No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAAGATCGGAAGAGCA264010.11673901042232053No Hit
TCTCGGCGCCCCCTCGATGCTTAGATCGGAAGAGCACACGTCTGAACTCC263100.11633662983262956Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAATAGATCGGAAGAGCAC259910.11492608688635024No Hit
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGATCGGAAGAGCACAC259130.11458118923804372No Hit
ACCTCCGCGTCGGTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA256980.11363050982283979Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCA251050.1110084033427657No Hit
CGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACACG245700.10864275921656057No Hit
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACA242750.10733833862360634No Hit
ACGGCCCTGGCGGAGCGCTGAGAAGACGGTCGAACTAGATCGGAAGAGCA239880.10606929214842716No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACAC235150.10397779743497851No Hit
TGTGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACACGTCTGAACTC230960.10212507801651131Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACGAGATCGGAAGAGCAC227420.10055977330496624No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)