FastQCFastQC Report
Fri 30 Jun 2023
SRR2007078.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2007078.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20873353
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA7800763.737185875216119No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA6998653.352911245260884No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC5395272.5847644123107583No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC4372152.0946083746104422No Hit
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC3008451.4412873676787816Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA2740261.3128029789943187No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC2021680.9685458776076847No Hit
TGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAAC2002310.9592661035340129Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC1596770.7649801160359814No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA1338000.6410086582639598No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG1189940.5700761157059913TruSeq Adapter, Index 4 (100% over 49bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC1052560.5042601445009817No Hit
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC1021520.4893895101568013Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAGATCGGAAG766990.3674493503750931No Hit
TCCCGTGGATCGCCTCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGT710580.3404244636690617Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACACGTCTGAACTCCA650150.3114736765099503Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG574680.275317530441803No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA571190.2736455422375121No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACTAGATCGGAAGAGCA547850.26246382169649507No Hit
TGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGC530190.25400327393495425No Hit
TCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCCA525880.25193844036461227Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA510750.2446899642812537No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC475110.2276155632494693No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA463690.22214447290763492No Hit
TGGGGGGAACCTCCGCGTCGGTAGATCGGAAGAGCACACGTCTGAACTCC436970.20934346293094355Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGATCGGAAGAGCACAC435170.20848111944448983No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA403150.19314098697990686No Hit
TATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACT383230.18359771906315195Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAG379980.18204070999038824No Hit
ACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT374790.17955428627111322Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCGGAGCGGGAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG356740.1709068974208408TruSeq Adapter, Index 4 (100% over 35bp)
TTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCAAGATCGGAAGAG353360.16928760798516654No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTC338850.16233616132491988TruSeq Adapter, Index 4 (100% over 50bp)
ACCACGAGGACGAGACGTAGCGTTCCCTCCTGAGCGAGATCGGAAGAGCA319110.15287912775681034No Hit
ACCGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCA318340.152510236376494No Hit
TCGCTGTGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC316300.15153291375851308No Hit
TGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGT315140.15097718128946508No Hit
CCGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCAC312370.1496501304797557No Hit
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACAC304590.14592289029941669No Hit
TCGCGTCGGGGTCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC274580.13154570806137375Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC265730.12730585258630944No Hit
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACAGATCGGAAGAGCACACGT262890.12594526619657131No Hit
CCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGC252930.12117363223819383No Hit
TCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC241550.1157217050849473TruSeq Adapter, Index 4 (100% over 37bp)
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAG224220.1074192536292564No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGAGATCGGAAGAGCA209670.10044864378042186No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)