FastQCFastQC Report
Fri 30 Jun 2023
SRR2007079.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2007079.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21371573
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC8491503.973268603111245No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA6903133.2300523690979603No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG4039541.890146317259848TruSeq Adapter, Index 9 (100% over 49bp)
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC3151911.4748142310348424No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA3138231.468413204774398No Hit
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC2945051.3780221044094414Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAAC2793591.307152262493734Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG2287761.070468701578494No Hit
TCCCGTGGATCGCCTCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGT1965400.9196328225348691Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATC1742880.8155132053218545TruSeq Adapter, Index 9 (100% over 37bp)
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC1469430.6875628668044228Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGCCCGTCACGTTGAACGCACGTTCGTGAGATCGGAAGAGCACACGTCT1350780.6320451938657019Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC1348870.6311514833278767No Hit
ACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG1340230.6271087299002278Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACATAGATCGGAAGAGCACA1267730.5931851623649789No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC1187640.5557101482422468No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA1068880.5001410050631275No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA924490.4325792958712024No Hit
TCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCCA904390.4231742792166024Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACTAGATCGGAAGAGCA890840.41683408142208345No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC877460.4105734285445437No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA850190.3978134880385267No Hit
TGGGGGGAACCTCCGCGTCGGTAGATCGGAAGAGCACACGTCTGAACTCC756830.35412929127865317Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACAGATCGGAAGAGCACACG749930.3509007034718502No Hit
TCGCCCGTCACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTCTGA631130.29531284384167694Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTC629140.29438170040174394TruSeq Adapter, Index 9 (100% over 50bp)
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAGATCGGAAGAGCACAC621640.290872365829132No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGAGATCGGAAGAGCA547510.2561861029134355No Hit
TGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACACGTCTGAACTCCA538870.25214334948578654Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACACG537940.2517081919987827No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA471370.22055933833227906No Hit
CGGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCT452790.2118655468177284TruSeq Adapter, Index 9 (100% over 43bp)
AAAACATTAGACTGTGAATCTGACAACAGGAGATCGGAAGAGCACACGTC439620.2057031553082218No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA404110.18908762588509512No Hit
TCTCGGCGCCCCCTCGATGCTCTAGATCGGAAGAGCACACGTCTGAACTC400100.1872113016669386Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCGTGGATCGCCTCAGTAGATCGGAAGAGCACACGTCTGAACTCCAGT368540.17244402178538754Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCAGATCGGAAG365700.17111515376055847No Hit
ACGGCCCTGGCGGAGCGCTGAGAAGACGGTCGAACTAGATCGGAAGAGCA351160.1643117238024548No Hit
GTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACT324520.15184656740053715Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGCCCGTCACGTTGAACGCACGTTCAGATCGGAAGAGCACACGTCTGAA320490.14996088495685367Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGC307490.1438780383643263No Hit
TGGGGGGAACCTCCGCGTCGGTAAGATCGGAAGAGCACACGTCTGAACTC302740.14165545980167205Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCCACCTATAACTTCTCTGTTAACCCAACACCGGAGATCGGAAGAGCAC295740.13838008086723425No Hit
TGTGGGGGGAACCTCCGCGTCGGTAAGATCGGAAGAGCACACGTCTGAAC287130.13435136477787574Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACAC284560.1331488327976607No Hit
TCCGGAGCGGGAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA284020.13289616070843263TruSeq Adapter, Index 9 (100% over 35bp)
AAACATTAGACTGTGAATCTGACAACAGGAGATCGGAAGAGCACACGTCT283940.13285872780632477Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA271250.12692093370946536TruSeq Adapter, Index 9 (100% over 35bp)
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAAGATCGGAAGAGCA270960.12678523943932438No Hit
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGATCGGAAGAGCACAC269970.1263220072757396No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACAC262910.12301855366472088No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCAC253000.11838155291610963No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAATAGATCGGAAGAGCAC252570.11818035106727989No Hit
TCGCTGTGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC251280.11757674552079064No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGAGATCGGAAGAGCACACG250190.11706672222957103No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATAGATCGGAAGAGCACACG250100.11702461021469968No Hit
TGTGGGGGGAACCTCCGCGTCGGTAGATCGGAAGAGCACACGTCTGAACT248580.11631338507465035Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA245710.11497047971153083No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACAC243980.11416099320344834No Hit
CTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACG239030.11184483238552445No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACGAGATCGGAAGAGCAC236200.11052064347345888No Hit
TCTCGGCGCCCCCTCGATGCTTAGATCGGAAGAGCACACGTCTGAACTCC233750.10937426084640564Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT229690.10747454106443172No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACGAGATCGGAAGAGCAC229550.10740903348574295No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC221000.10340839207296534No Hit
TCCGCGCGGCGCCTCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC219630.10276735362436823Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCGGAGCGGGAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG217820.10192043421417787Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)