FastQCFastQC Report
Fri 30 Jun 2023
SRR2007080.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2007080.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21983441
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA5966452.7140655550693817No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA5340882.429501368780256No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC5142772.3393835387280815No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC3616211.6449699571600278No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA2914051.3255659111783273No Hit
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC2688501.2229659587868889Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACTAGATCGGAAGAGCA2467831.1225858590563689No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC2256051.0262497122265801No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC2217041.0085045375744406No Hit
TGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAAC1789420.813985399283033Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC1573260.7156568437125016No Hit
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC1479390.6729565221386407Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG1470980.6691309154012787TruSeq Adapter, Index 5 (100% over 49bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA1120440.5096745318442185No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG1040060.47311064723670876No Hit
TGGGGGGAACCTCCGCGTCGGTAGATCGGAAGAGCACACGTCTGAACTCC900760.40974477107564733Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC898400.4086712357724161No Hit
TCCCGTGGATCGCCTCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGT842240.38312473465823665Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA789090.35894744594351724No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACATAGATCGGAAGAGCACA764350.34769352077320376No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACACG635140.28891746292129605No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT611870.2783322228763004No Hit
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACAC603420.2744884206253243No Hit
TGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGC562650.2559426433741651No Hit
TGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACACGTCTGAACTCCA551630.2509297793734839Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACG533430.24265082067907387No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAATAGATCGGAAGAGCAC504950.22969561498584323No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAAGATCGGAAGAGCA463340.2107677319487882No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA461070.20973513655118867No Hit
TCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCCA458340.2084932927470272Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGT437940.1992135808038423No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCAC437780.19914079874938595No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA424960.19330913663607077No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACAGATCGGAAGAGCAC389890.1773562200749191No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTC360000.16375962252679188TruSeq Adapter, Index 5 (100% over 50bp)
TCCGGAGCGGGAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC342770.15592190503752348TruSeq Adapter, Index 5 (100% over 35bp)
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAACAGATCGGAAGAGCAC340010.15466641459815142No Hit
TCTCGGCGCCCCCTCGATGCTTAGATCGGAAGAGCACACGTCTGAACTCC334290.15206445615133682Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC325480.14805689427783394No Hit
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACA318670.14495910808503548No Hit
TCGCGTCGGGGTCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC312770.14227526982695748Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGGGGGGAACCTCCGCGTCGGTAAGATCGGAAGAGCACACGTCTGAACTC291550.1326225498546838Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGAGGCCCTGTAATTGGAATGAGTCCACTTTAGATCGGAAGAGCACACG291140.1324360458401394No Hit
TATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACT290070.13194931585096256Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCGTGGATCGCCTCAGTAGATCGGAAGAGCACACGTCTGAACTCCAGT283700.12905168030791905Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA279520.12715024913524683Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA277870.1263996841986657No Hit
TCTCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC265920.12096377450645693No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACAC251880.11457714922791205No Hit
TCGCTTCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC249300.11340353859980337No Hit
TCGCTGTGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC242020.11009195512203937No Hit
ACGGAGGTAGAAGAAAGGGCTCTGGCCCTCTCGGCGTTAGATCGGAAGAG228570.10397371366930228No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)