FastQCFastQC Report
Fri 30 Jun 2023
SRR2007081.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2007081.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22835371
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG8464673.706823944309904TruSeq Adapter, Index 10 (100% over 49bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC6539492.8637546550042914No Hit
TGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAAC6205472.7174815771550196Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC5128892.246028759506469Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA3537731.549232548050128No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA3397611.4878716005971613No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC3057421.3388965740911327No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG2344591.0267361103964545No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA2278050.9975971049474082No Hit
ACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT2221120.9726664830626137Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC1943270.8509912100836899No Hit
TCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGC1371960.6008047778159593TruSeq Adapter, Index 10 (100% over 37bp)
TCCCGTGGATCGCCTCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGT1357910.5946520422199403Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC1326970.5811028863949703Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACACGTCTGAACTCCA1220040.5342764082965852Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC1035460.4534456654984935No Hit
CGGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCT944430.41358206967603023TruSeq Adapter, Index 10 (100% over 43bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTC937440.4105210289773702TruSeq Adapter, Index 10 (100% over 50bp)
TGGGGGGAACCTCCGCGTCGGTAGATCGGAAGAGCACACGTCTGAACTCC805930.35293054796438383Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA785430.3439532469168117No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGAGATCGGAAGAGCACACGTCT779510.34136077754112254Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA667470.29229654293770835No Hit
TCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCCA656190.28735683777592225Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGAGATCGGAAGAGCA610870.26751043370392363No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAGATCGGAAGAGCACAC537340.23531038755621705No Hit
TCCGGAGCGGGAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA527700.23108886647823676TruSeq Adapter, Index 10 (100% over 35bp)
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA502860.22021100511132488No Hit
GTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACT489090.21418088631010196Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA480250.21030969893153914TruSeq Adapter, Index 10 (100% over 35bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA467780.2048488723918696No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC466380.20423578841788906No Hit
AAAACATTAGACTGTGAATCTGACAACAGGAGATCGGAAGAGCACACGTC433470.18982393585810364No Hit
TGTGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACACGTCTGAACTC420150.18399087976280307Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA411500.18020289663785186No Hit
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACAC399720.17504423291392987No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACAGATCGGAAGAGCACACG377360.1652524060152121No Hit
TTGGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATC324740.1422092069360292TruSeq Adapter, Index 10 (100% over 42bp)
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACTAGATCGGAAGAGCA316060.13840808629734985No Hit
TCGCCCGTCACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTCTGA315390.13811468182408773Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGATCGGAAGAGCACAC290340.12714485786107876No Hit
TGTGGGGGGAACCTCCGCGTCGGTAGATCGGAAGAGCACACGTCTGAACT288770.126457327975972Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC286520.1254720144463604No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGAGATCGGAAGA281580.12330870385245767No Hit
TCCGGAGCGGGAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT277730.12162272292401118Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGC266230.1165866759948853No Hit
TGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG263660.11546122898550674TruSeq Adapter, Index 10 (100% over 49bp)
AGACAACACTGACCTGGACACTTGGGCTTCTGCGTGAGATCGGAAGAGCA260900.11425257772251653No Hit
CTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACG252740.11067917398845853No Hit
AAAAACATTAGACTGTGAATCTGACAACAGGAGATCGGAAGAGCACACGT243920.10681674495238111No Hit
TCGCTGTGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC231450.10135591841271158No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)