FastQCFastQC Report
Fri 30 Jun 2023
SRR2007082.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2007082.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20677901
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA4376922.116713877293445No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA2827831.3675614367241626No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC2723111.31691799859183No Hit
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC2359171.140913673974936Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC1816090.8782757979158524No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA1343050.6495098317764457No Hit
TGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAAC1247050.6030834561012745Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC1185500.5733173787803705No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACTAGATCGGAAGAGCA1034430.5002587061423691No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC945610.4573046364812367No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC929190.4493637918084626No Hit
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC784130.37921160373095897Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC721690.3490151152188996No Hit
TCCCGTGGATCGCCTCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGT687960.3327030146821962Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGGGGGGAACCTCCGCGTCGGTAGATCGGAAGAGCACACGTCTGAACTCC650640.3146547611384734Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACACG620790.30021905995197484No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA593930.28722934692452584No Hit
TCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCCA492580.23821566802162364Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA461920.22338824429036583No Hit
TGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACACGTCTGAACTCCA455050.2200658567811114Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG422000.20408260973877376TruSeq Adapter, Index 1 (100% over 49bp)
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACAC411630.19906759394969537No Hit
TCGAGGCCCTGTAATTGGAATGAGTCCACTTTAGATCGGAAGAGCACACG409650.19811004995139497No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT360300.17424399120587725No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG351490.16998340402152037No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA291730.14108298516372625No Hit
AGGCCCTGTAATTGGAATGAGTCCACTTTAGATCGGAAGAGCACACGTCT275730.13334525588453103Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACATTAGACTGTGAATCTGACAACAGGAGATCGGAAGAGCACACGTCT268160.12968434271931178Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCAGATCGGAAGAGC247380.119634966817957No Hit
TTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCAAGATCGGAAGAG246780.11934480196998719No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA241620.11684938427744672No Hit
TCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA240140.11613364431912118TruSeq Adapter, Index 1 (100% over 37bp)
TCGCGTCGGGGTCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC232170.11227928792192204Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACATAGATCGGAAGAGCACA221100.10692574647687887No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAAGATCGGAAGAGCA215950.10443516486513792No Hit
ACCACGAGGACGAGACGTAGCGTTCCCTCCTGAGCGAGATCGGAAGAGCA214880.10391770421959172No Hit
TCCGGAGCGGGAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT210010.10156253287023669TruSeq Adapter, Index 1 (100% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)