FastQCFastQC Report
Fri 30 Jun 2023
SRR2007084.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2007084.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16251551
Total Bases828.8 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAAC1882601.1584125109043437Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC1879081.1562465637895116No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG1878371.1558096824112356TruSeq Adapter, Index 6 (100% over 49bp)
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC1363180.8387999397719025Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA863270.531192376653773No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTC779180.47944962299290694TruSeq Adapter, Index 6 (100% over 50bp)
TCGCTGCGATCTATTGAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT611760.3764317633436956Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA552580.34001677747557757No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC511350.31464689124133444No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA444420.273463129765276No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC421400.2592983278949806No Hit
TCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCA368950.2270244852322095TruSeq Adapter, Index 6 (100% over 37bp)
TCGCTGCGATCTATTGAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC334020.20553115207280834Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA322430.198399525066869No Hit
TGAGAGTGATGATTTCACAGACTAGAGTCTCTGACGAGATCGGAAGAGCA303710.1868806245016245No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC303570.18679447887773912No Hit
TGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGC229010.14091578089992765No Hit
ACGACATGGAGAAGATCTGGCACCACACCTTCTACAAGATCGGAAGAGCA185350.11405065276538837No Hit
TCGCGTCGGGGTCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC167140.10284556840144059Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)