FastQCFastQC Report
Sun 2 Jul 2023
SRR2007085.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2007085.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18983118
Total Bases968.1 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG7437553.9179812294271152TruSeq Adapter, Index 2 (100% over 49bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTC4972392.6193747518189583TruSeq Adapter, Index 2 (100% over 50bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC4688862.4700157266050815No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC1122610.5913728187329395No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA988980.5209786927521601No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA841530.44330441395349274No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG727230.38309301980844246No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACAC647820.34126111421737987No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACA623230.3283074993265068No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA617960.3255313484328549No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTAGATCGGAAGAGCAC596740.3143529951191369No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGAGATCGGAAGAGCAC469300.24721966117473432No Hit
CTGGACTCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGT429060.22602187901903156TruSeq Adapter, Index 2 (100% over 39bp)
ATATTAAATGGATTTTTGGAAGTAGGAGTTGGAGATCGGAAGAGCACACG349070.18388443879451205No Hit
ATGGTTAGCACTCTGGACTCAGATCGGAAGAGCACACGTCTGAACTCCAG323010.17015645164298088Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTCGGCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTAT321130.16916609800349974TruSeq Adapter, Index 2 (100% over 41bp)
TGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAAC320730.16895538446318462Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC294230.15499561241730678No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACAC288660.15206142636841852No Hit
TGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGC258480.13616308975164143No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG251460.13246506711911077No Hit
AATTCCGATAACGAACGAGACTCTGGCATGCTAGATCGGAAGAGCACACG247510.1303842709084988No Hit
CGGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT239880.12636491012698756TruSeq Adapter, Index 2 (100% over 43bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACG231210.12179769414065698No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACAGATCGGAAGAGCAC221220.11653512347128644No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT216190.1138854007018236No Hit
ATTCGGCTGATCTGGCTGGCTAGGCGGGTGAGATCGGAAGAGCACACGTC212430.11190469342286129No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTAGATCGGAAGAGCACA209260.11023478861586385No Hit
ATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGATCGGAAGAGCAC207670.10939720229311117No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCAGATCGGAAGAGCA197960.10428213110196122No Hit
AATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGATCGGAAGAGCA189960.10006786029565849No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)