FastQCFastQC Report
Fri 30 Jun 2023
SRR2007087.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2007087.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17567181
Total Bases895.9 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG2134521.2150611984928033TruSeq Adapter, Index 3 (100% over 49bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC1513280.861424493776207No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTC1208470.6879134449630819TruSeq Adapter, Index 3 (100% over 50bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG900350.5125182008428103No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA831150.4731265648142408No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC727630.4141984988940457No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC622660.3544450301957952No Hit
ATATTAAATGGATTTTTGGAAGTAGGAGTTGGAGATCGGAAGAGCACACG586870.33407181266020997No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA571630.32539654484120134No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAGATCGGAAGAGCACA521890.29708238333742903No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA503030.28634645478975823No Hit
ATATTAAATGGATTTTTGGAAGTAGGAGTTGGAAGATCGGAAGAGCACAC350690.19962793119738448No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTAGATCGGAAGAGCAC347930.1980568197026034No Hit
CGGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCT328620.18706473167208784TruSeq Adapter, Index 3 (100% over 43bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG307720.17516754680218757No Hit
TATATTAAATGGATTTTTGGAAGTAGGAGTTGGAGATCGGAAGAGCACAC303250.17262302927259643No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC256400.1459539808919826No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG248060.1412064918099267No Hit
TGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGC217220.12365102858563363No Hit
TATATTAAATGGATTTTTGGAAGTAGGAGTTGGAAGATCGGAAGAGCACA206200.11737796747241347No Hit
ACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT205700.1170933458248082Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATTAAATGGATTTTTGGAAGTAGGAGTTGGAGATCGGAAGAGCACA197910.11265894055511809No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC182250.10374459055212103No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)