FastQCFastQC Report
Fri 30 Jun 2023
SRR2007091.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2007091.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17489985
Total Bases891.9 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG2887901.6511735144426938No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG2095711.198234303803005TruSeq Adapter, Index 5 (100% over 49bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC1887031.0789203078218763No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC1365200.7805609896177726No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTC1255250.7176964417064966TruSeq Adapter, Index 5 (100% over 50bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAGATCGGAAGAGCACA1158730.6625105739084396No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG775920.44363674411384574No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC764610.43717018625230375No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA645350.3689825920376718No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC462140.2642312157500421No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA440320.2517555046502327No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATAGATCGGAAGAGCACACG290780.16625514544466446No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA260510.14894809801151915No Hit
ATATTAAATGGATTTTTGGAAGTAGGAGTTGGAGATCGGAAGAGCACACG257500.1472271131164492No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTAGATCGGAAGAGCACAC255480.14607216644268134No Hit
ACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA236880.13543750895155143Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAGATCGGAAGAGCACACG220930.12631800427501796No Hit
ATATTAAATGGATTTTTGGAAGTAGGAGTTGGAAGATCGGAAGAGCACAC219770.12565476757127006No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC210990.12063475183083348No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTAGATCGGAAGAGCAC208540.11923395017205561No Hit
CGGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCT205290.11737574389000334TruSeq Adapter, Index 5 (100% over 43bp)
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACAC201120.1149915222911855No Hit
TCGAGGCCCTGTAATTGGAATGAGTCCACTTTAGATCGGAAGAGCACACG200450.11460844591919318No Hit
GTTTCTGTAGTGTAGTGGTTATCACGCTCGCCTGAGATCGGAAGAGCACA195180.11159529296337303No Hit
GGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCA190260.10878225453023545No Hit
GTTTCCGTAGTGTAGTGGTCATCACGCTCGCCTGAGATCGGAAGAGCACA182950.10460271978506558No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACAGATCGGAAGAGCAC180280.10307613185488725No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG179660.10272164327184957No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)