FastQCFastQC Report
Fri 30 Jun 2023
SRR2007092.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2007092.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15210074
Total Bases775.7 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC3731832.453525209673536No Hit
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC588890.38717102888519805Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC569040.37412046778996605No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG532130.34985365620180414TruSeq Adapter, Index 10 (100% over 49bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA437460.2876120129330074No Hit
TGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAAC409460.269203161010262Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA405390.2665273028914915No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC379160.2492821533938625No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA356140.23414744727737682No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG339870.22345058939226728No Hit
CCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGC273410.17975586443563654No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA270890.17809906776258946No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTC239130.15721817001021823TruSeq Adapter, Index 10 (100% over 50bp)
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGATCGGAAGAGCACAC227930.14985462924112006No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA212790.13990070002289273No Hit
TCTGCTCTGGTCGAGGTTGGCGGTTGAGGGTGAGATCGGAAGAGCACACG197420.12979555523529995No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC172580.11346427374383583No Hit
TATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACT171940.11304349998560163Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)