FastQCFastQC Report
Fri 30 Jun 2023
SRR2007093.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2007093.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17142687
Total Bases874.2 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG3337031.9466201535383574TruSeq Adapter, Index 1 (100% over 49bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC2456961.4332408915825157No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC1493110.8709894779039015No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA1413450.8245206833677824No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG1270610.7411965230421579No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTC778270.45399533923707525TruSeq Adapter, Index 1 (100% over 50bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA726850.42400004153374554No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC671230.3915547195139245No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAGATCGGAAGAGCACA561540.32756825111489235No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA443910.25895007007944554No Hit
ACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA438460.2557708718592365Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCT423480.2470324517970841TruSeq Adapter, Index 1 (100% over 43bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG391830.22856976855495292No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG363880.212265440067826No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTAGATCGGAAGAGCAC357020.20826373368422346No Hit
TGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAAC289060.1686200068869017Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC279170.16285078296068756Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACAGATCGGAAGAGCAC262090.15288735074028942No Hit
TCTCCGCTCCCACTGCTTCACTTGACCAGCCTTAGATCGGAAGAGCACAC242970.14173390670902408No Hit
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC231430.13500217322990263Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCAC228450.13326382264343975No Hit
TCTCGGCGCCCCCTCGATGCTCTAGATCGGAAGAGCACACGTCTGAACTC222950.13005545746708203Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC202790.1182953407479236No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC195500.1140427985414422No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACAC186440.10875774608729658No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)