FastQCFastQC Report
Fri 30 Jun 2023
SRR2007094.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2007094.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20026160
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC15939627.959399105969392No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG5729602.8610577364806833No Hit
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC3647231.8212328274616802Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA1501430.7497343474735046No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA1436480.7173017692857742No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA1431240.714685191769166No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG1064930.5317694455652007No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG1017970.5083201172865892TruSeq Adapter, Index 2 (100% over 49bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC950990.47487386498460016No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC639170.31916752887223515No Hit
TCGCTGTGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC625550.31236642471647086No Hit
ACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC595230.2972262280936535Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAAC535770.26753506413610995Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA519850.2595854622154222No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA472910.23614612087389691No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT472390.23588646050965337No Hit
TCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT463660.2315271624714873TruSeq Adapter, Index 2 (100% over 37bp)
TGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA432700.21606738386190863No Hit
TATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACT356120.17782740175849987Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAGATCGGAAGAGCACACG348520.17403236566570923No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC344920.17223471699017687No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC320180.15988087581443472No Hit
TCGCTGCGATCTATTGAAAGTCAGTCCTCGACACAAGATCGGAAGAGCAC315510.15754892600478573No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACAC271810.13572746847123962No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAGATCGGAAGAGCACA265640.1326464983801188No Hit
ATATTAAATGGATTTTTGGAAGTAGGAGTTGGAGATCGGAAGAGCACACG236100.1178957923036668No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT223600.11165395662473485No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC221620.11066524985319202No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACAC220840.11027575930682668No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCA212970.10634589956337111No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACA209620.10467308760141733No Hit
CCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGC201610.10067331929835775No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)