FastQCFastQC Report
Fri 30 Jun 2023
SRR2007096.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2007096.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23577690
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC4259791.806703710159901No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG2088240.8856847299290134No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG2005500.8505922335903136TruSeq Adapter, Index 3 (100% over 49bp)
TGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCAGATCGGAAGAGCAC1517560.6436423585177343No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC1193140.5060461817930425No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCA1144510.48542075156641723No Hit
ATATTAAATGGATTTTTGGAAGTAGGAGTTGGAGATCGGAAGAGCACACG998990.4237013888977249No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAGATCGGAAGAGCACA973930.4130726971132456No Hit
TGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCAGATCGGAAGAGCACA806510.3420648927015327No Hit
ATTCCGTAGAGCATGCCTGTTTGAGTGCCGTGAGATCGGAAGAGCACACG763940.3240096888202364No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA741050.3143013586148601No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCAGATCGGAAGAGCA664760.2819444992278718No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC600600.254732333829141No Hit
ATATTAAATGGATTTTTGGAAGTAGGAGTTGGAAGATCGGAAGAGCACAC582790.2471785828043375No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA531600.22546738039222672No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA527670.22380055043560249No Hit
TATATTAAATGGATTTTTGGAAGTAGGAGTTGGAGATCGGAAGAGCACAC457800.19416660410752706No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG436210.1850096425900926No Hit
TGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACAC381560.16183095120853655No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA368780.15641057287630808No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTAGATCGGA332800.1411503841131171No Hit
TATATTAAATGGATTTTTGGAAGTAGGAGTTGGAAGATCGGAAGAGCACA304220.1290287555735952No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCAGATCGGAAGAGCAC285500.12108904646723237No Hit
ATATATTAAATGGATTTTTGGAAGTAGGAGTTGGAGATCGGAAGAGCACA280190.11883691744186983No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC279430.11851457882430382No Hit
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC273870.11615641735895246Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAAC254120.1077798545998357Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGC240590.10204137894764076No Hit
TGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAGATCGGAAGAGC239120.10141790820050649No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)