FastQCFastQC Report
Fri 30 Jun 2023
SRR2007098.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2007098.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21716584
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG9109434.194688262205511TruSeq Adapter, Index 4 (100% over 49bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG5139032.3664080870177373No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC1609950.7413458764969665No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA1049700.4833633134935034No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAGATCGGAAGAGCACA974590.448776842619447No Hit
CGGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCT940710.4331758622811027TruSeq Adapter, Index 4 (100% over 43bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA937120.431522747776538No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG887370.408613988277346No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC847050.3900475323374984No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTC790970.36422394977036904TruSeq Adapter, Index 4 (100% over 50bp)
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC556690.25634326282623454Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC510630.23513366558939472No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA477930.2200760487929409No Hit
TATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACT472300.21748356002951477Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA466670.21489107126608864No Hit
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC465910.21454110830690498Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAGATCGGAAGAGCACACG405260.1866131432088951No Hit
TCGCGTCGGGGTCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC396290.18248265933537244Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTGGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATC392440.18070982066056063TruSeq Adapter, Index 4 (100% over 42bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC379220.17462230708107684No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCAGATCGGAAGAGCAC337080.15521778194949998No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT320410.14754162072635363No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG299820.13806038739794435No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG296250.136416482444937No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGGACTCTGAGATCGGAAGAGCA292720.1347909965950446No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT275470.12684775837673182No Hit
TGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGT274220.1262721614043903No Hit
CGCGTCGGGGTCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA260010.11972877502281205Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC241780.11133426877818352No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTAGATCGGAAGAGCAC231870.10677093598146006No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT220920.10172870650374848No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)