FastQCFastQC Report
Fri 30 Jun 2023
SRR2007099.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2007099.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23696982
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC9515854.015637940730174No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA2821851.1908056477402904No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA1919580.8100525206121185No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA1814820.7658443594209592No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG1176330.49640498524242455TruSeq Adapter, Index 9 (100% over 49bp)
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC940220.3967678246959887No Hit
TGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAAC864680.3648903476400497Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC707850.29870892419971456Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGC669840.2826689069519486No Hit
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGATCGGAAGAGCACAC568270.23980690874475072No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC516540.21797712468195318No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCA438620.1850953003213658No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACTAGATCGGAAGAGCA394920.16665413342509186No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC336130.1418450670216148No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC290240.12247973180719805No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACATAGATCGGAAGAGCACA274950.11602743336683127No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTAAGATCGGAAGAGCACA249090.1051146513087616No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)