FastQCFastQC Report
Sat 1 Jul 2023
SRR2007101.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2007101.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20573167
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC9700564.71515153695102No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA2444511.1882030608121736No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA1752110.85164816870441No Hit
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC1606630.7809347000391335Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA1565470.7609280574060376No Hit
CCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGC950610.4620630357980373No Hit
TGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAAC930380.4522298389936756Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG729690.3546804437061149TruSeq Adapter, Index 10 (100% over 49bp)
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGATCGGAAGAGCACAC685170.33304060575603167No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC495080.24064355283753835No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCA464440.22575036697072454No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC452910.22014597946927666No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA316700.1539383800267601No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC310470.15091016370984595No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC296770.1442510042328437No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)