FastQCFastQC Report
Fri 30 Jun 2023
SRR2007102.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2007102.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20689853
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC5257182.5409460376543036No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG4300912.078753290320622No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC3155851.5253129154663398No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC2921001.4118031674753804No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA2074671.002747578728568No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA1453560.7025472824770674No Hit
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC1371550.6629094948137138Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATATTAAATGGATTTTTGGAAGTAGGAGTTGGAGATCGGAAGAGCACACG814640.3937389018665334No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG646580.31251067854372866No Hit
TATATTAAATGGATTTTTGGAAGTAGGAGTTGGAGATCGGAAGAGCACAC535110.25863402702764493No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA498140.24076536454850594No Hit
ACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA474400.22929114092787414Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATAGATCGGAAGAGCACACG443890.21454478192764348No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC433860.2096969949472333No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA416940.20151907314179565No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA405080.1957867946185988No Hit
ATATATTAAATGGATTTTTGGAAGTAGGAGTTGGAGATCGGAAGAGCACA402890.19472830473952618No Hit
TGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAAC399420.19305115410921478Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACAGATCGGAAGAGCAC382980.18510523008549168No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACGAGATCGGAAGAGCAC354190.17119019646973807No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG339790.16423026301830176TruSeq Adapter, Index 1 (100% over 49bp)
TCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA327090.15809198837710448TruSeq Adapter, Index 1 (100% over 37bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACAC317630.15351969876248034No Hit
GGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCA299100.14456361773087514No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGC297270.14367912618808842No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG291680.1409773186885378No Hit
TCGCTGTGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC283450.13699952338955718No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTCAGATCGGAAGAGC282210.1364001957867946No Hit
TATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACT279440.13506137525481696Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT266340.12872976912885753No Hit
ATATATTAAATGGATTTTTGGAAGTAGGAGTAGATCGGAAGAGCACACGT239240.11563156103622388No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC228350.11036811136357519No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACG226280.1093676209299312No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCAGATCGGAAGAGCA225210.10885045920819253No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC217820.10527866002721237No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAGATCGGAAGAGCACA217580.10516266113635511No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACA216890.10482916432514044No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAGATCGGAAGAGCACACG210600.10178902672725611No Hit
TGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGT210180.10158602866825589No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)