FastQCFastQC Report
Fri 30 Jun 2023
SRR2007769.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2007769.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17607850
Total Bases898 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG353772720.091760209224862No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC189080810.738437685464154No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA2456811.3952924405875788No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA2325881.3209335608833561No Hit
TATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACT1332140.756560284191426Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT1138200.6464162291250778No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAGATCGGAAGAGCACACG1108750.6296907345303373No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG1033530.5869711520713772No Hit
GACCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG1019450.5789747186624148No Hit
GATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTG925440.5255837595163521Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG775250.44028657672572175TruSeq Adapter, Index 3 (100% over 49bp)
GTTTCCGTAGTGTAGTGGTCATCACGCTCGCCTGAGATCGGAAGAGCACA766270.4351865787134716No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC756540.42966063431935186No Hit
CTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAAC751230.4266449339357162Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC679170.3857200055656994No Hit
GGCTCTGTAGCTCAGTAGGTAGAGCAACGGTAGATCGGAAGAGCACACGT672780.3820909423921717No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACAC629030.35724407011645376No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC622190.35335943911380435No Hit
TGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGT587400.33360120628015344No Hit
GTTTCTGTAGTGTAGTGGTTATCACGCTCGCCTGAGATCGGAAGAGCACA583270.33125566153732566No Hit
GCCCGACTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG570570.324042969471003No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATAGATCGGAAGAGCACACG554200.3147459797760658No Hit
TCGCTGCGATCTATTGAAAGTCAGATCGGAAGAGCACACGTCTGAACTCC550870.3128547778405654Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC526300.29890077437052226No Hit
AGCCGATTTAGCTCAGCGGTAGAGCAGCTGGCAGATCGGAAGAGCACACG513340.29154042089181814No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG513020.29135868376888713No Hit
TCGCTGCGATCTATTGAAAGTAGATCGGAAGAGCACACGTCTGAACTCCA484830.27534877909568745Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCCGGCTAACTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG481600.273514370011103No Hit
GTTTCCGTAGTGTAGTGGTCATCACGCTCGCCTAGATCGGAAGAGCACAC422710.24006906010671375No Hit
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGATCGGAAGAGCACAC419050.23799044176319087No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACA406240.23071527756086063No Hit
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC403240.22901149203338284No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAGATCGGAAGAGCACA402670.22868777278316205No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA400050.22719980008916477No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA393580.2235253026349043No Hit
TAGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC380730.2162274212922077No Hit
CGGACACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATC379420.2154834349452091TruSeq Adapter, Index 3 (100% over 42bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGAGATCGGAAGAGCACA377800.2145633907603711No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACAC370590.21046862620933277No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC362800.20604446312298208No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC346720.19691217269570105No Hit
TCGCTGCGATCTATTGAAAGAGATCGGAAGAGCACACGTCTGAACTCCAG342830.1947029307950715Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCCGATTTAGCTCAGCGGTAGAGCAGCTGGCTGAGATCGGAAGAGCACAC335580.19058544910366684No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACAGATCGGAAGAGCACACG335350.1904548255465602No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG319210.18128845940872965No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCA317900.180544473061731No Hit
GTTTCCGTAGTGTAGTGGTCATCACGCTCGCCAGATCGGAAGAGCACACG316880.17996518598238856No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGGCAGATCGGAAGAGCACACG316040.17948812603469474No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC308320.17510371794398522No Hit
CTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACG301440.17119636980096944No Hit
GCCAGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG290230.16482989121329406No Hit
ATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGA289740.16455160624380602Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC282740.16057610667969116No Hit
TCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG278440.1581340140903063No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT276380.15696408136143822No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT274340.15580550720275332No Hit
ATGGTTAGCACTCTGGACTCAGATCGGAAGAGCACACGTCTGAACTCCAG273950.1555840150841812Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC269700.15317031892025432No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC268710.15260806969618665No Hit
GCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTC267200.15175049764735615No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACA254420.1444923713003007No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAGATCGGAAGAGCACAC244810.13903457832728014No Hit
TCCTCCCGACCCCTCCACCCGCGCGTCGTTCCCCAGATCGGAAGAGCACA244600.13891531334035673No Hit
TCCTCCCGACCCCTCCACCCGCGCGTCGTTCCCCTCAGATCGGAAGAGCA243650.13837578125665542No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG236430.1342753374205255No Hit
TCGCTGCGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGG225750.12820986094270453TruSeq Adapter, Index 3 (100% over 38bp)
TCGCTGCGATCTATTGAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT224810.12767600814409483Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG223080.1266934918232493No Hit
TCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAA222480.12635273471775374Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA222140.12615963902463959No Hit
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACAC219370.12458647705426841No Hit
TCCGGAGCGGGAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT206010.11699895217190061Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACAC203680.11567567874555952No Hit
TCGCTGCGATCTATTGAAAGTCAAGATCGGAAGAGCACACGTCTGAACTC202840.11519861879786572Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT194600.11051888788239335Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCCGTCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG191080.10851977953015275No Hit
CTCGACACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCA189110.10740096036710899TruSeq Adapter, Index 3 (100% over 40bp)
CTCGCTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCAT187520.10649795403754575TruSeq Adapter, Index 3 (100% over 41bp)
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACA183600.10427167428164143No Hit
TCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTG182760.10379461433394764Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTTCTGTAGTGTAGTGGTTATCACGCTCGCCAGATCGGAAGAGCACACG178020.10110263320053273No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)