FastQCFastQC Report
Fri 30 Jun 2023
SRR2007770.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2007770.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8004515
Total Bases408.2 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC7979489.96872390144812No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG3577104.468852891149557No Hit
TATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACT2330202.9111070439620637Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA2231672.7880140145905155No Hit
ATTGTTTAATGGGGGTAGAGCACTGAATGAGATCGGAAGAGCACACGTCT1937232.4201716156444206Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTCTGTAGCTCAGTAGGTAGAGCAACGGTAGATCGGAAGAGCACACGT1838242.296503910605452No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC1382651.7273376338229112No Hit
AGCTGTACTGACTATAATCAAACTCCGAATACCATTAGATCGGAAGAGCA1027151.2832132865014307No Hit
GCCGATTTAGCTCAGCGGTAGAGCAGCTGGCAGATCGGAAGAGCACACGT954481.192427023998331No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC946071.1819204536439747No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA845961.0568535382843307No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA844671.0552419478256958No Hit
TAGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC741760.9266770066643637No Hit
ATTGTTTAATGGGGGTAGAGCACTGAATGTGGAGATCGGAAGAGCACACG740570.9251903456986463No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC684540.8551923508170076No Hit
AGCCGATTTAGCTCAGCGGTAGAGCAGCTGGCAGATCGGAAGAGCACACG550860.6881866046849809No Hit
ATGGGGGTAGAGCACTGAATGTGGAATGGCGGCAGATCGGAAGAGCACAC473920.5920658528343066No Hit
GACCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG464240.579972677919899No Hit
TCTCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC386910.4833647010468467No Hit
ATTGTTTAATGGGGGTAGAGCACTGAATGTGAGATCGGAAGAGCACACGT382140.47740556423468505No Hit
ATTGTTTAATGGGGGTAGAGCACTGAATAGATCGGAAGAGCACACGTCTG377770.4719461453941931Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTG362360.4526945105356165Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC356320.44514876916340346No Hit
CTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAAC347990.4347421424033811Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCGATTTAGCTCAGCGGTAGAGCAGCTGGCTGAGATCGGAAGAGCACAC342130.4274212741184194No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA335380.41898853334649255No Hit
TGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGT326580.40799473796975827No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT302880.3783864481483263No Hit
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC298090.37240232543758117Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATTGTTTAATGGGGGTAGAGCACTGAATGTAGATCGGAAGAGCACACGTC269310.3364476173759435No Hit
ATGGGGGTAGAGCACTGAATGTGGAATGGCGGCAAGATCGGAAGAGCACA266560.333012056320714No Hit
TATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACACGTCTGAACTC262140.3274901727337634Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCCGATTTAGCTCAGCGGTAGAGCAGCTGGAGATCGGAAGAGCACACGT258380.32279282380006785No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA248830.3108620572264528No Hit
ATCTTGAGTTGTGATGCATAGTGAAGACTTCGGTCGAGATCGGAAGAGCA231500.2892117761038614No Hit
TAGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA207750.25954102153597064No Hit
GGGAGCGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA183890.2297328445258707TruSeq Adapter, Index 3 (100% over 36bp)
GGCTCTGTAGCTCAGTAGGTAGAGCAACGGCAGATCGGAAGAGCACACGT175950.2198134427882264No Hit
ACGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC173610.21689009265395842No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACA168470.2104687167180023No Hit
GCCGATTTAGCTCAGCGGTAGAGCAGCTGGCTAGATCGGAAGAGCACACG163480.2042347350214223No Hit
TCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAA163120.20378498884691953Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCTCTGTAGCTCAGTAGGTAGAGCAACGGTAGATCGGAAGAGCACACGT156110.19502743139340736No Hit
ACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT155710.1945277134217376Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACT155270.1939780236529009Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGGGACTCAAACACAATACCGAAGCTACGGGCACAAGATCGGAAGAGCA155080.19374065761635775No Hit
ATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGA153330.19155439149030265Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAGATCGGAAGAGCACACG146900.18352142509571162No Hit
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACAC143080.1787491184662656No Hit
GGCTCTGTAGCTCAGTAGGTAGAGCAACGGAGATCGGAAGAGCACACGTC139220.1739268400396526No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT138440.17295238999489662No Hit
CCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC134640.1682050692640341No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC127930.15982230028927424No Hit
TCGCTGCGATCTATTGAAAGTAGATCGGAAGAGCACACGTCTGAACTCCA126420.15793586494622097Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGCGGAGTAGAGCAGTTGGAAGCTCGTTGGGCAGATCGGAAGAGCACACG126100.15753609056888518No Hit
TCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG123340.15408803656436398No Hit
TTTGTTTAATGGGGGTAGAGCACTGAATGAGATCGGAAGAGCACACGTCT121000.151164686430096Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC118300.14779159012132526No Hit
GACGATTTAGCTCAGCGGTAGAGCAGCTGGCAGATCGGAAGAGCACACGT116120.1450681271757252No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG115760.14461838100122243No Hit
ATGGACAACAGGTTAATATTCCTGTACTTGGTAGATCGGAAGAGCACACG112850.1409829327573251No Hit
ATTGTTTAATGGGGGTAGAGCACTAGATCGGAAGAGCACACGTCTGAACT112540.14059565132928104Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACTGAATGTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGG110520.13807207557234885TruSeq Adapter, Index 3 (100% over 38bp)
TATCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC110410.13793465313013967No Hit
TCTCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA106990.1336620644723634No Hit
ACCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC106340.13285002276840008No Hit
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC106150.13261265673185696Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGCTGCGATCTATTGAAAGTCAGATCGGAAGAGCACACGTCTGAACTCC104710.1308136720338459Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA100270.1252668025483118No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC99360.12412994416276313No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCA97650.121993649833875No Hit
TGGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC97130.12134401647070435No Hit
CTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACG93620.1169589912693024No Hit
ACTGTTTAATGGGGGTAGAGCACTGAATGAGATCGGAAGAGCACACGTCT90930.1135983879098234Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACAGATCGGAAGAGCACACG90770.11339850072115551No Hit
TAGCTGTACTGACTATAATCAAACTCCGAATACCATTAGATCGGAAGAGC90500.11306119109027843No Hit
AATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACT90170.11264892376365088Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC87430.10922585565771319No Hit
TCGCTGCGATCTATTGAAAGAGATCGGAAGAGCACACGTCTGAACTCCAG86470.1080265325257058Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT86170.10765174404695349No Hit
GGCTCTGTAGCTCAGTAGGTAGAGCAACGGTTAGATCGGAAGAGCACACG85820.10721449082174248No Hit
GACCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC85640.1069896177344911No Hit
AACGGGACTCAAACACAATACCGAAGCTACGGGCACAAGATCGGAAGAGC84040.10499074584781215No Hit
TTGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC81620.10196745211921023No Hit
TCGCTGCGATCTATTGAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT81580.10191748032204326Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA80810.100955523226579No Hit
GGGAGCGTAGCTCAGATGGTTAGAGCACACGACAGATCGGAAGAGCACAC80560.1006431994942854No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)