FastQCFastQC Report
Fri 30 Jun 2023
SRR2007771.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2007771.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34274786
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC24918987.2703531978288645No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC22225176.484408101045474No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA16826984.909434007844717No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC11886413.4679749714556936No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA10618653.0980937415626753No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA9494732.7701792215420395No Hit
AGCTGTACTGACTATAATCAAACTCCGAATACCATTAGATCGGAAGAGCA8447032.464502623006895No Hit
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACAC5198141.5166075726920658No Hit
ATGGGGGTAGAGCACTGAATGTGGAATGGCGGCAGATCGGAAGAGCACAC3726751.0873153226981491No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA3006460.8771637553039718No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT1953010.5698095387087172No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC1918000.559595032920118No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG1797750.5245109334891253No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAACAGATCGGAAGAGCAC1642470.47920649307628066No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACTAGATCGGAAGAGCA1635640.4772137745805327No Hit
AGCGGAGTAGAGCAGTTGGAAGCTCGTTGGGCAGATCGGAAGAGCACACG1264340.3688834118468311No Hit
ATGGGGGTAGAGCACTGAATGTGGAATGGCGGCAAGATCGGAAGAGCACA1027080.29966051429175955No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAGATCGGAAGAGCACAC1006310.29360066609898017No Hit
AGGAGCCAATGGGGCGAAGCTACCATCTGTGGGAAGATCGGAAGAGCACA1000220.2918238497535769No Hit
ACCGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCA931070.27164866908286456No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACATAGATCGGAAGAGCACA812500.2370547258850865No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA776560.22656888361024338No Hit
AACGGAGAGAGGTGGTATCCCCGGTGGCGAGATCGGAAGAGCACACGTCT753620.2198759169495617Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTCAGATCGGAAGAGCACAC721760.21058045409824No Hit
AGGAGCCAATGGGGCGAAGCTACCATCTGTGGGATAGATCGGAAGAGCAC698600.2038233003117802No Hit
ATGGGGGTAGAGCACTGAATGTGGAATGGCGGTAGATCGGAAGAGCACAC683880.199528598077899No Hit
AGCTGTACTGACTATAATCAAACTCCGAATACCATCAGATCGGAAGAGCA681220.19875251737530908No Hit
ACGGGACTCAAACACAATACCGAAGCTACGGGCACAAGATCGGAAGAGCA649270.18943079615435088No Hit
TCTCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC640370.18683413515696348No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCA605460.17664880533462704No Hit
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATCGGAAGAGCACACG567850.16567572442319553No Hit
TATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACT567600.16560278450753857Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC560710.16359256043203305No Hit
TGGGGCGAAGCTACCATCTGTGGGATTATGACTGAGATCGGAAGAGCACA554990.16192369516180202No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACA554500.16178073292711442No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAATAGATCGGAAGAGCAC543100.1584546727731575No Hit
AGCCGATTTAGCTCAGCGGTAGAGCAGCTGGCAGATCGGAAGAGCACACG538810.15720302382048423No Hit
TAGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC526580.15363480314654626No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACAC524690.15308337738417974No Hit
GGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCAC514160.150011148136709No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAAGATCGGAAGAGCA470160.13717372298108585No Hit
ATGGACAACAGGTTAATATTCCTGTACTTGGTAGATCGGAAGAGCACACG465640.1358549693060082No Hit
GGCTCTGTAGCTCAGTTGGTTAGAGCGTTCGGAGATCGGAAGAGCACACG463440.13521309804822704No Hit
CTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAAC463310.13517516929208545Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGCTCTGTAGCTCAGTAGGTAGAGCAACGGTAGATCGGAAGAGCACACGT445650.1300226936500785No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACGAGATCGGAAGAGCAC440570.1285405545639293No Hit
ACCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA422620.12330346861976031No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA413150.12054050461467505No Hit
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACAGATCGGAAGAGCACACGT406950.11873159470638271No Hit
ACCGGTCGGTCACTGGTTCGAGTCCAGTTGGGTAGATCGGAAGAGCACAC399600.11658716118606838No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAAGATCGGAAGAGCACAC397120.11586359722275145No Hit
ACCGGTCGGTCACTGGTTCGAGTCCAGTTGGGTCCAGATCGGAAGAGCAC396230.11560393112301269No Hit
ACGGGACTCAAACACAATACCGAAGCTACGGGCACAGATCGGAAGAGCAC390090.11381252679447801No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACAC387450.11304228128514063No Hit
AGCCGATTTAGCTCAGCGGTAGAGCAGCTGGAGATCGGAAGAGCACACGT387270.11298976454586762No Hit
ACTCCGGATGTGCTGACCCCTGCGATTTCCCCAGATCGGAAGAGCACACG384000.11203571044907472No Hit
AACGGGACTCAAACACAATACCGAAGCTACGGGCACAGATCGGAAGAGCA376100.10973080911431511No Hit
GATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTG373940.10910060824303906Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCTTGAGTTGTGATGCATAGTGAAGACTTCGGTCGAGATCGGAAGAGCA370780.10817864770913523No Hit
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGATCGGAAGAGCACAC344610.10054329733816574No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)