FastQCFastQC Report
Sat 1 Jul 2023
SRR2007772.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2007772.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44962240
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA19871244.41953959589202No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC11485222.554414548741344No Hit
AGCTGTACTGACTATAATCAAACTCCGAATACCATTAGATCGGAAGAGCA10080042.241890083768068No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA8747971.9456259296689846No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC7937851.7654480737614497No Hit
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACAC7131721.586157629157266No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC5703621.2685355533887992No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC5431591.2080336744788516No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA5306081.180119139971674No Hit
ACCGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCA4963471.1039196445728683No Hit
GGCTCTGTAGCTCAGTAGGTAGAGCAACGGTAGATCGGAAGAGCACACGT4200300.9341838840769499No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA4031510.8966434946301607No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAGATCGGAAGAGCACAC3970450.8830632103738604No Hit
ATGGGGGTAGAGCACTGAATGTGGAATGGCGGCAGATCGGAAGAGCACAC3848290.8558937455073412No Hit
GGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCAC2823050.6278712982271345No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG2709390.6025923085682564No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA2611430.5808051378223149No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACTAGATCGGAAGAGCA2126290.47290570932409065No Hit
ATTGTTTAATGGGGGTAGAGCACTGAATGAGATCGGAAGAGCACACGTCT1959600.4358323784580127Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGGAGTAGAGCAGTTGGAAGCTCGTTGGGCAGATCGGAAGAGCACACG1660160.3692342730255432No Hit
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATCGGAAGAGCACACG1659400.36906524230109533No Hit
ATTGTTTAATGGGGGTAGAGCACTGAATGTGGAGATCGGAAGAGCACACG1572730.3497890674485969No Hit
AGGAGCCAATGGGGCGAAGCTACCATCTGTGGGAAGATCGGAAGAGCACA1517550.33751654721828805No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG1510290.3359018589821148TruSeq Adapter, Index 8 (100% over 49bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT1474390.3279173813404314No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA1438060.31983726789412625No Hit
ATTGTTTAATGGGGGTAGAGCACTGAATGTGAGATCGGAAGAGCACACGT1408660.3132984477641683No Hit
AGGAGCCAATGGGGCGAAGCTACCATCTGTGGGATAGATCGGAAGAGCAC1381320.3072177898610034No Hit
ATGGGGGTAGAGCACTGAATGTGGAATGGCGGCAAGATCGGAAGAGCACA1315170.292505444568598No Hit
ACCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA1304060.2900344822677874No Hit
AACGGAGAGAGGTGGTATCCCCGGTGGCGAGATCGGAAGAGCACACGTCT1068980.23775061028987882Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC1057650.23523071804251744No Hit
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTCAGATCGGAAGAGCACAC1045880.2326129659020547No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCAC1017580.2263187955048503No Hit
GGCTCTGTAGCTCAGTTGGTTAGAGCGTTCGGAGATCGGAAGAGCACACG985140.21910385247710076No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAACAGATCGGAAGAGCAC914510.2033951155458447No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA901600.20052381731871008No Hit
AGCTGTACTGACTATAATCAAACTCCGAATACCATCAGATCGGAAGAGCA887060.19728999266940436No Hit
ACCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC862330.19178982185940913No Hit
ATGGGGGTAGAGCACTGAATGTGGAATGGCGGTAGATCGGAAGAGCACAC860510.19138503775612603No Hit
TAGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA836750.18610060352864982No Hit
TCTTAGATCGATGTGGTGCTCCGGAGTTCTCAGATCGGAAGAGCACACGT778440.17313194360423326No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACAC774940.1723535126363811No Hit
CCGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCAC767380.17067210174582048No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC754380.1677807867223697No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG749030.16659089938579574No Hit
ATTGTTTAATGGGGGTAGAGCACTGAATGTAGATCGGAAGAGCACACGTC743540.16536987481050766No Hit
TGTAGTATCTGTTCTTATCAGTTTAATATCTGATAGATCGGAAGAGCACA738310.1642066765356886No Hit
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGATCGGAAGAGCACAC732560.16292782565993155No Hit
ACACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACAC729330.162209445081028No Hit
TATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACT705840.15698506124250036Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGAGGTGTAGTTCAATGGTAGAACAACGGGCTTCAGATCGGAAGAGCACA692190.15394918046787706No Hit
ACCTGTACTGACTATAATCAAACTCCGAATACCATTAGATCGGAAGAGCA690820.15364448034617492No Hit
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC674600.15003700883230017Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT658210.1463917278142726No Hit
ACGGGACTCAAACACAATACCGAAGCTACGGGCACAAGATCGGAAGAGCA649570.14447011536791762No Hit
ATTGTTTAATGGGGGTAGAGCACTGAATAGATCGGAAGAGCACACGTCTG636130.14148094045136542Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCTCTGTAGCTCAGTAGGTAGAGCAACGGCAGATCGGAAGAGCACACGT610030.13567606951966807No Hit
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACAGATCGGAAGAGCACACGT583260.129722184659839No Hit
GGCTCTGTAGCTCAGTAGGTAGAGCAACGGTTAGATCGGAAGAGCACACG576110.12813196139694108No Hit
TGCTGTACTGACTATAATCAAACTCCGAATACCATTAGATCGGAAGAGCA567870.1262993124897692No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAATAGATCGGAAGAGCAC549880.12229817731500921No Hit
TGGGGCGAAGCTACCATCTGTGGGATTATGACTGAGATCGGAAGAGCACA540670.12024979182531832No Hit
TAGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC535380.1190732490196218No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA511360.11373098849167657No Hit
ATCTTGAGTTGTGATGCATAGTGAAGACTTCGGTCGAGATCGGAAGAGCA510850.11361755997921812No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACAC495640.1102347214017807No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAAGATCGGAAGAGCACAC476660.10601340146754255No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC474240.10547517205548478No Hit
TCTCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA466090.1036625399446291No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC462280.10281516223391006No Hit
AGCGGAGTAGAGCAGTTGGAAGCTCGTTGGGTAGATCGGAAGAGCACACG458840.10205007579693538No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)