FastQCFastQC Report
Sat 1 Jul 2023
SRR2007773.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2007773.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60458951
Total Bases3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG1330642022.0090156708144No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC794314313.138076113824734No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA8813251.4577245973056991No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA8164571.3504319649872853No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG6665441.1024736436462486No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACAC6308491.043433585210567No Hit
TATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACT5623850.9301931156562739Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GACCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG3980100.6583144322169929No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAGATCGGAAGAGCACACG3618960.5985813415783545No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT2964140.490273144170166No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC2955300.48881099508325904No Hit
CTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAAC2859140.4729059887261359Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACAC2767450.4577403269864871No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC2546920.42126433850961126No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC2413800.3992460934361895No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC2234960.3696656926779957No Hit
GATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTG2224030.36785785449701236Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC2090230.34572713641690545No Hit
GCCCGACTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG1873800.30992929400974883No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT1820570.3011249732070277No Hit
GTTTCCGTAGTGTAGTGGTCATCACGCTCGCCTGAGATCGGAAGAGCACA1805340.2986059086602412No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACA1751470.2896957309100517No Hit
TAGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC1695680.280467982317457No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC1674210.2769168125328539No Hit
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGATCGGAAGAGCACAC1563320.25857544236915386No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG1527020.25257136862993207No Hit
AGCCGATTTAGCTCAGCGGTAGAGCAGCTGGCAGATCGGAAGAGCACACG1514090.2504327274881101No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATAGATCGGAAGAGCACACG1435050.23735939447576587No Hit
AGCGGAGTAGAGCAGTTGGAAGCTCGTTGGGCAGATCGGAAGAGCACACG1419700.2348204817513291No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC1406340.2326107179729268No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGGCAGATCGGAAGAGCACACG1361270.22515607325042739No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA1357750.22457386003935131No Hit
GCCAGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG1335990.2209747238254266No Hit
TCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG1328930.2198069893736661No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG1303710.2156355640374905No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC1298110.21470931574714222No Hit
GCCCGGCTAACTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG1296770.21448767776338032No Hit
GTTTCTGTAGTGTAGTGGTTATCACGCTCGCCTGAGATCGGAAGAGCACA1270210.21009461444344277No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCA1218300.20150862359487515No Hit
GTTTCCGTAGTGTAGTGGTCATCACGCTCGCCTAGATCGGAAGAGCACAC1169970.1935147700462087No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG1132160.1872609400715537No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG1092200.18065149691399707TruSeq Adapter, Index 4 (100% over 49bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAGATCGGAAGAGCACACG1061890.17563817804248705No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACAGATCGGAAGAGCACACG1060810.17545954444363415No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA1044020.17268245358739354No Hit
GGCTCTGTAGCTCAGTAGGTAGAGCAACGGTAGATCGGAAGAGCACACGT1034390.17108963733095534No Hit
TGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGT1003360.1659572293935434No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC969870.1604179338142999No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC951840.1574357451223393No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAGATCGGAAGAGCACA921150.1523595736882699No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG919870.15214785979333317No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTAGATCGGAAGAGCACACG884040.1462215247499084No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGAGATCGGAAGAGCACA857330.141803651207908No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA853300.14113708324181806No Hit
GTTTCCGTAGTGTAGTGGTCATCACGCTCGCCAGATCGGAAGAGCACACG823210.1361601526960003No Hit
ATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGA821030.13579957746868615Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGCTGCGATCTATTGAAAGTAGATCGGAAGAGCACACGTCTGAACTCCA798610.13209127627768466Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACA797310.13187625435313954No Hit
TCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAA793540.13125269077195864Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACAC791320.13088549948542771No Hit
GCCGATTTAGCTCAGCGGTAGAGCAGCTGGCTGAGATCGGAAGAGCACAC785970.13000060156518428No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT747340.12361114237658541No Hit
CGGACACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATC745860.12336634818556479TruSeq Adapter, Index 4 (100% over 42bp)
CTCGCTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAAT742530.1228155612557684TruSeq Adapter, Index 4 (100% over 41bp)
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACAC725060.11992599739284263No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACGAGATCGGAAGAGCAC723880.11973082364594781No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACG699730.11573637789382088No Hit
TCGCTGCGATCTATTGAAAGTCAGATCGGAAGAGCACACGTCTGAACTCC679270.1123522636044413Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGAACTGGTGCGGACCAGGGGAATCCGACTGAGATCGGAAGAGCACACGT644940.10667403078164556No Hit
CCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC644580.1066144862486946No Hit
TCCTCCCGACCCCTCCACCCGCGCGTCGTTCCCCAGATCGGAAGAGCACA632570.10462801446885837No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAGATCGGAAGAGCACAC625320.1034288537358182No Hit
AGAACGAAAGTCGGAGGTTCGAAGACGATCAGAGATCGGAAGAGCACACG618010.10221976891395287No Hit
CTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACG610480.10097429576639529No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)