FastQCFastQC Report
Fri 30 Jun 2023
SRR2007774.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2007774.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5600537
Total Bases285.6 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC4814628.596711351072228No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC4399987.8563537746469665No Hit
TATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACT3463546.184299826963022Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA2449204.373152074524282No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA1871123.340965339573687No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC1489942.660352034099587No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA1381302.4663706355301285No Hit
TGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGT1020361.8218967216893664No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA828971.480161634500406No Hit
GATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTG731991.3069996680675442Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACACGTCTGAACTC508060.9071630095471203Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAAC498880.8907717242114461Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC465940.8319559356540276No Hit
TAGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC418160.7466426880136673No Hit
TCGCTGCGATCTATTGAAAGTAGATCGGAAGAGCACACGTCTGAACTCCA265830.4746509129392414Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC244010.4356903632633799No Hit
TCTCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC238720.4262448404501211No Hit
GATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACT235630.4207275123796164Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACG227290.4058360832184485No Hit
TCCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA226670.4047290465182178No Hit
GCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC221370.39526566827431014No Hit
TCGCTGCGATCTATTGAAAGTCAGATCGGAAGAGCACACGTCTGAACTCC215350.3845166990236829Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG213650.3814812758133729No Hit
TCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAA204590.36530425564548547Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGA204480.3651078459083477Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC194940.34807376506931387No Hit
TAGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA178200.31818377416308474No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA168680.3011854041853486No Hit
GCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTC168610.3010604161708065No Hit
TCGCTGCGATCTATTGAAAGAGATCGGAAGAGCACACGTCTGAACTCCAG162180.2895793742635751Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACT135600.2421196395988456Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA131290.23442394898917726No Hit
GGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA128160.2288351991960771No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCAC128020.22858522316699273No Hit
GATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACACGTCTGA120800.21569360223849962Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACACGTC115280.20583740452031654No Hit
ACGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC105140.18773199784234976No Hit
ACGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC101310.1808933679038278No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC100680.1797684757729482No Hit
TCTCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA100390.1792506682841306No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACAC98740.17630452222706502No Hit
TCGCTGCGATCTATTGAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT96520.17234061662301312Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC96300.1719477971487377No Hit
CTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACACGTCTGAACT92760.1656269746990333Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAGATCGGAAGAGCACAC89540.15987752603009317No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC88220.15752060918444072No Hit
CCCAAAGTCGGTGAGTTAACTTCGGAGACCATTGAGATCGGAAGAGCACA88070.15725277772470747No Hit
CATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACT86130.15378882417882428Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC85390.15246752231080699No Hit
GCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA84160.15027130434099445No Hit
CCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC82180.1467359290725157No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC80420.14359337327831243No Hit
AGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA77970.1392187927693362No Hit
ATGGGGGTAGAGCACTGAATGTGGAATGGCGGCAGATCGGAAGAGCACAC75960.13562985120891086No Hit
TCCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGT75660.1350941882894444No Hit
AGCTGTACTGACTATAATCAAACTCCGAATACCATTAGATCGGAAGAGCA75260.1343799710634891No Hit
GAGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC74830.13361218754558715No Hit
TAGTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACT73980.13209447594043214Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACAC73540.1313088369918813No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAAAGATCGGAAGAGCACA72380.12923760703661094No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAAGATCGGAAGAGCACAC71760.12813057033638026No Hit
TATCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC71000.12677355760706518No Hit
TCTCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA70740.12630931641019424No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACA67260.12009562654438316No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTAGATCGGAAGAGCACAC64890.11586388948059802No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC63810.11393550297051872No Hit
TATCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC63790.11389979210922095No Hit
GAGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC63210.11286417713158577No Hit
GCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA61840.11041798313268888No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCA58630.10468638989439762No Hit
ATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACTC58250.1040078835297401Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCT57710.10304369027470044Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC57020.10181166555992754No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)