FastQCFastQC Report
Sat 1 Jul 2023
SRR2007775.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2007775.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29433660
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC21473057.295406007951441No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC18537286.29798672676113No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA15563535.287663851522373No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA15157505.1497163451640064No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC14939255.0755665452410605No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA10552203.5850791236971546No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA5214971.7717708229285791No Hit
AGCTGTACTGACTATAATCAAACTCCGAATACCATTAGATCGGAAGAGCA4741011.6107442975151578No Hit
ATGGGGGTAGAGCACTGAATGTGGAATGGCGGCAGATCGGAAGAGCACAC3733751.2685306550391626No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG1620830.5506722575445935No Hit
AGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAGATCGGAAGAGCACAC1519600.5162796607693368No Hit
TATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACT1336150.4539530591846206Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGCTCTGTAGCTCAGTAGGTAGAGCAACGGTAGATCGGAAGAGCACACGT1335590.4537628008205572No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC1205380.4095243337050167No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAGATCGGAAGAGCACAC1186000.4029400353201063No Hit
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACAC1111810.37773419955248516No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA978590.33247309373010353No Hit
ATGGGGGTAGAGCACTGAATGTGGAATGGCGGCAAGATCGGAAGAGCACA929230.3157031779262246No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT927780.3152105446621317No Hit
ACCGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCA896730.3046613978689704No Hit
CTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAAC884580.3005334708629508Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCAGATCGGAAGAG842810.28634223538628906No Hit
GATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTG792640.26929712444867543Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCTTGAGTTGTGATGCATAGTGAAGACTTCGGTCGAGATCGGAAGAGCA734660.249598588826534No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCA640550.21762499125151272No Hit
ACCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC636420.21622183581654475No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAACAGATCGGAAGAGCAC575240.1954361095426121No Hit
TCTCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC572750.1945901393166871No Hit
TGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGT553080.18790731427895818No Hit
ACCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA530390.18019845306360133No Hit
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGATCGGAAGAGCACAC496020.16852134596920668No Hit
TCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTG484320.16454630514859517Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC469060.15936176472786598No Hit
ACTCCGGATGTGCTGACCCCTGCGATTTCCCCAGATCGGAAGAGCACACG466990.15865848827498857No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACA458760.1558623698174131No Hit
ACGGACCAAGGAGTCTAACGCGTGCGCGAGTCAGATCGGAAGAGCACACG458100.1556381367454812No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCAC455060.15460530562627958No Hit
ACGGGACTCAAACACAATACCGAAGCTACGGGCACAAGATCGGAAGAGCA414020.1406620855170577No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACTAGATCGGAAGAGCA405870.13789314682577702No Hit
TCTCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA374520.1272420759090103No Hit
TCTCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC366700.12458525375369559No Hit
AGCCGATTTAGCTCAGCGGTAGAGCAGCTGGCAGATCGGAAGAGCACACG364780.12393293936262088No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA354690.12050489133869181No Hit
AGCTGTACTGACTATAATCAAACTCCGAATACCATTAAGATCGGAAGAGC353660.12015495184764655No Hit
AACGGGACTCAAACACAATACCGAAGCTACGGGCACAAGATCGGAAGAGC353480.12009379737348327No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCAC349650.11879256606212071No Hit
GGCTCTGTAGCTCAGTTGGTTAGAGCGTTCGGAGATCGGAAGAGCACACG348080.11825916314858566No Hit
TCGCTGCGATCTATTGAAAGTCAGATCGGAAGAGCACACGTCTGAACTCC341180.11591490830566092Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCCGATTTAGCTCAGCGGTAGAGCAGCTGGAGATCGGAAGAGCACACGT337100.11452874022462717No Hit
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACAGATCGGAAGAGCACACGT326900.11106332002204279No Hit
AATGGGGGTAGAGCACTGAATGTGGAATGGCGGCAGATCGGAAGAGCACA322720.10964317723314056No Hit
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC319050.10839630545436754Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGCTGCGATCTATTGAAAGTAGATCGGAAGAGCACACGTCTGAACTCCA318240.1081211103206329Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAGTGTAGTATCTGTTCTTATCAGTTTAATATCTGAAGATCGGAAGAGCA317190.10776437588801394No Hit
ATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGA317020.10770661888463752Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA310570.10551525022712091No Hit
TCGCTGCGATCTATTGAAAGAGATCGGAAGAGCACACGTCTGAACTCCAG306990.10429895568542953Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC305450.10377574518425504No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCAC304830.10356510199547048No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC304260.10337144616062019No Hit
CTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACG296270.10065686700192909No Hit
ACGGGACTCAAACACAATACCGAAGCTACGGGCACAGATCGGAAGAGCAC294350.10000455261085439No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)