FastQCFastQC Report
Fri 30 Jun 2023
SRR2007776.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2007776.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27741791
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC286124910.313858250896635No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA9009133.24749400642518No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCAC6224682.2437916859801876No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG6149252.2166016606498116TruSeq Adapter, Index 9 (100% over 49bp)
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA5547291.9996149491573922No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA4920861.7738076103305658No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAGATCGGAAGAGCACAC3178321.145679455230558No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA2956001.0655404332041865No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA2255070.8128783033510705No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACAC2053020.7400459472858115No Hit
GGCTCTGTAGCTCAGTTGGTTAGAGCGTTCGGAGATCGGAAGAGCACACG2043070.736459300699079No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCACA1979330.7134831345243716No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC1949410.7026979620746188No Hit
TCTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC1807910.6516918824743507No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACAC1712760.6173934480293648No Hit
GTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC1557550.5614453659462721No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC1476420.5322006787521397No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC1406770.5070941526450112No Hit
ACCGGTCGGTCACTGGTTCGAGTCCAGTTGGGTCCAGATCGGAAGAGCAC1370330.4939587353967161No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT1366100.4924339600136126No Hit
ACCGGAGTGGACGTACCGCTGGTGTTCCAGTAGATCGGAAGAGCACACGT1234970.44516592313740666No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT1125840.405828160121313No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA1084090.39077866313678167No Hit
AGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGATCGGAAGAGCACA1065530.3840883957347959No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC1062240.3829024593257155No Hit
CGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACACG995620.35888814820932075No Hit
AGCCGATTTAGCTCAGCGGTAGAGCAGCTGGAGATCGGAAGAGCACACGT838050.30208936402123426No Hit
ATTGTTTAATGGGGGTAGAGCACTGAATGAGATCGGAAGAGCACACGTCT798360.2877824290436043Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGGTCGGTCACTGGTTCGAGTCCAGTTGGGTCTAGATCGGAAGAGCAC797050.28731021728193396No Hit
ATTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC791470.28529881145741454No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC790940.2851077639507846No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC756610.2727329320590729No Hit
CTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC753590.2716443217382757No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA742300.2675746493800635No Hit
ACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG733740.26448905191449246Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGGGACTCAAACACAATACCGAAGCTACGGGCACAAGATCGGAAGAGCA707830.255149352109242No Hit
AAGGCGAGCGAGAAAACCAATGTTAAGGAACTCAGATCGGAAGAGCACAC680330.24523650978410155No Hit
TTGGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC637480.22979049910656452No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACATAGATCGGAAGAGCACA632770.22809269956651318No Hit
TCGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA608630.21939102634000812No Hit
TTTGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA569350.20523188282977117No Hit
AACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACACGT564420.2034547805511187No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC521850.18810970063180132No Hit
ACCGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCA501890.18091477943871756No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA499800.18016140342200687No Hit
AACGGGACTCAAACACAATACCGAAGCTACGGGCACAAGATCGGAAGAGC489140.17631882526978881No Hit
ATTGTTTAATGGGGGTAGAGCACTGAATAGATCGGAAGAGCACACGTCTG483100.17414160462819434Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGGTCGGTCACTGGTTCGAGTCCAGTTGGGTCAGATCGGAAGAGCACA481950.17372706758550666No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCACAC472600.17035670119495888No Hit
AAGGCGAGCGAGAAAACCAATGTTAAGGAACAGATCGGAAGAGCACACGT466890.16829843466126612No Hit
ATGGACAACAGGTTAATATTCCTGTACTTGGTAGATCGGAAGAGCACACG464110.16729633641894281No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAGATCGGAAGAGCAC453120.16333480415882307No Hit
AAGGCGAGCGAGAAAACCAATGTTAAGGAACTAGATCGGAAGAGCACACG433990.15643907057046172No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGAGATCGGAAGAGCACAC433750.1563525584919878No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCACA429620.1548638298082485No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG426990.15391580161497143No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCGTCCGCCAGATCGGAAGAGCA418960.15102125165603042No Hit
AGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAGATCGGAAGAGCACAC414440.149391940844771No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT405140.14603959780390532No Hit
GTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA400410.14433458892398116No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC399510.14401016862970384No Hit
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACAC396260.14283865090036907No Hit
TCTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCAC384800.1387076991532378No Hit
AAAAAACCGATCTCAGTTCGGATTGAAGTCTGAGATCGGAAGAGCACACG364730.1314731265908535No Hit
TCCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCACCAGATCGGAAGA356270.12842357582464667No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACAC346640.1249522786758793No Hit
GTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCAC336820.12141249279832005No Hit
TGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG327480.11804573107770872TruSeq Adapter, Index 9 (100% over 49bp)
ATTGTTTAATGGGGGTAGAGCACTGAATGTAGATCGGAAGAGCACACGTC326890.11783305555146024No Hit
TATCTGATGTGGGCGTTGGAATATTGATGAGAGCAGATCGGAAGAGCACA326730.11777538083247761No Hit
TCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATC325950.11749421657743728TruSeq Adapter, Index 9 (100% over 37bp)
AGAACTGGTGCGGACCAGGGGAATCCGACTGTCAGATCGGAAGAGCACAC318420.114779900115317No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACAGATCGGAAGAGCACACG302130.10890789278889745No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGT298990.10777602642886323No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACGAGATCGGAAGAGCAC292270.10535368823159255No Hit
TAATCAAACTCCGAATACCATTAGATCGGAAGAGCACACGTCTGAACTCC291000.10489589514966788Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACG288990.10417135649244853No Hit
TGGACAACAGGTTAATATTCCTGTACTTGGTAGATCGGAAGAGCACACGT287730.10371716808046028No Hit
ACCGGTCGGTCACTGGTTCGAGTCCAGTTGGGTAGATCGGAAGAGCACAC287190.10352251590389387No Hit
AACGGGACTCAAACACAATACCGAAGCTACGGGCACAGATCGGAAGAGCA287040.10346844585484767No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA285770.103010652772923No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAGATCGGAAGAGCACACG282280.10175262296511427No Hit
TAGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA281380.10142820267083692No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)