FastQCFastQC Report
Fri 30 Jun 2023
SRR2007778.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2007778.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7300567
Total Bases372.3 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC5128507.0247968411220665No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA2111292.8919534606010737No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG2010842.7543614078194203No Hit
TATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACT1900912.603784062251603Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC1746982.3929374252712154No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC919881.2600117223771796No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA833061.1410894523671928No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA583090.7986913893126384No Hit
TAGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC482120.6603870630870178No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC409990.5615865178690915No Hit
TATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACACGTCTGAACTC360110.49326305751320415Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA358640.49124951527737504No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACAC305570.4185565312940762No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG297940.4081052882604872No Hit
TCTCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC286330.3922024138673065No Hit
CTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAAC276190.37831308170995487Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GACCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG274220.3756146611626193No Hit
TGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGT246390.33749433434416803No Hit
GCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC238160.3262212373367712No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG230480.31570150647203155No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC228060.3123866954443401No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA220980.30268881855340823No Hit
GATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTG216690.29681256264068256Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA209360.2867722465939974No Hit
GGCTCTGTAGCTCAGTAGGTAGAGCAACGGTAGATCGGAAGAGCACACGT197260.27019819145554036No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACAC196490.2691434788558204No Hit
TCGCTGCGATCTATTGAAAGTAGATCGGAAGAGCACACGTCTGAACTCCA174870.23952934066627973Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC173480.2376253789602917No Hit
AGCTGTACTGACTATAATCAAACTCCGAATACCATTAGATCGGAAGAGCA172470.2362419247710486No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG164860.22581807686991984No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC161500.22121569461659624No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACATAGATCGGAAGAGCACA157380.21557229732978275No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT150230.20577853747523991No Hit
GTTTCCGTAGTGTAGTGGTCATCACGCTCGCCAGATCGGAAGAGCACACG139990.19175222965558703No Hit
TCGCCCGTCACGTTGAACGCACGTTAGATCGGAAGAGCACACGTCTGAAC129020.17672599950113463Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACA116100.15902874393180694No Hit
ACGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC115730.15852193398129213No Hit
TCTCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA115410.158083611861928No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT112710.15438526897979293No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTAGATCGGAAGAGCACAC109540.1500431404848418No Hit
TTGGAATGAGTCCACTTTAAATCCTTTAACGAGGAGATCGGAAGAGCACA108600.14875556925920957No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT107580.14735841750373635No Hit
ATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGA104830.14359158679045067Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT103670.1420026691077556No Hit
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC103400.14163283481954209Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAA101400.13889332157351614Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCAGATCGGAAGAGCACACGTCTGA101320.1387837410436751Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC101240.13867416051383405No Hit
GTTTCTGTAGTGTAGTGGTTATCACGCTCGCCAGATCGGAAGAGCACACG100030.13701675499998836No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATAGATCGGAAGAGCACACG98890.13545523244975355No Hit
GCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA98290.13463337847594575No Hit
TCGCTGCGATCTATTGAAAGTCAGATCGGAAGAGCACACGTCTGAACTCC98130.1344142174162637Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATTGTTTAATGGGGGTAGAGCACTGAATGAGATCGGAAGAGCACACGTCT97590.1336745488398367Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC94220.12905846902028292No Hit
TCTCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC94070.12885300552683102No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAGATCGGAAGAGCACAC92450.12663399979754997No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC88540.12127825140156921No Hit
TACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCA86560.11856613328800351Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACAC86200.11807302090371885No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAGATCGGAAGAGCACACG84080.11516913686293133No Hit
AAAAAGGGGTTGTAGGACATCAGATCGGAAGAGCACACGTCTGAACTCCA83630.11455274638257548Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACGAGATCGGAAGAGCAC82500.11300492139857082No Hit
GCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC81520.11166255990801811No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG80720.11056675460960773No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG80180.1098270860331807No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACG79570.10899153449314279No Hit
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC77900.10670404093271113Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAAGATCGGAAGAGCACAC76610.10493705488902438No Hit
AGCCGATTTAGCTCAGCGGTAGAGCAGCTGGCAGATCGGAAGAGCACACG74010.10137568766919064No Hit
CCCAAAGTCGGTGAGTTAACTTCGGAGACCATTGAGATCGGAAGAGCACA73750.10101955094720726No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)