FastQCFastQC Report
Fri 30 Jun 2023
SRR2007779.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2007779.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17578993
Total Bases896.5 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC8652614.922130636265684No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC4884872.7788110502120342No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA4023452.2887829809136395No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA2972741.6910752510112494No Hit
TATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACT2899301.64929811394771Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGCTCTGTAGCTCAGTAGGTAGAGCAACGGTAGATCGGAAGAGCACACGT2696671.5340298502877838No Hit
ATGGGGGTAGAGCACTGAATGTGGAATGGCGGCAGATCGGAAGAGCACAC2617211.4888281712154956No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA2012581.1448778664397898No Hit
AGCCGATTTAGCTCAGCGGTAGAGCAGCTGGCAGATCGGAAGAGCACACG1742760.9913878457087957No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA1553250.883583035729066No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACAC1435280.8164745272951641No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC1416630.8058652733976287No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG1388190.7896868722798855No Hit
AGCTGTACTGACTATAATCAAACTCCGAATACCATTAGATCGGAAGAGCA1266170.7202744776108619No Hit
AGCGGAGTAGAGCAGTTGGAAGCTCGTTGGGCAGATCGGAAGAGCACACG1179780.6711305932029212No Hit
CTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAAC1108170.6303944714011775Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTG1060690.6033849606743685Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCGATTTAGCTCAGCGGTAGAGCAGCTGGAGATCGGAAGAGCACACGT1029850.5858412936395162No Hit
GCCGATTTAGCTCAGCGGTAGAGCAGCTGGCAGATCGGAAGAGCACACGT766740.43616832886843976No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCT685470.3899370117503318Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGATTTAGCTCAGCGGTAGAGCAGCTGGCTGAGATCGGAAGAGCACAC678370.3858981000788839No Hit
TGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGT608090.34591856313953817No Hit
TATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACACGTCTGAACTC579550.32968327594191543Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC531530.3023665803837569No Hit
GGCTCTGTAGCTCAGTTGGTTAGAGCGTTCGGAGATCGGAAGAGCACACG517910.2946186963041626No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC448630.2552080201636123No Hit
CTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACG422330.24024698115529142No Hit
ACCGGTCGGTCACTGGTTCGAGTCCAGTTGGGTCAGATCGGAAGAGCACA385120.21907967083211194No Hit
ACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG365260.20778209536803388Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACA364870.20756023965650366No Hit
GGGAGCGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGC353920.20133121390969325TruSeq Adapter, Index 11 (100% over 36bp)
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGAAGAGCA350890.199607565689343No Hit
TCGCTGCGATCTATTGAAAGTAGATCGGAAGAGCACACGTCTGAACTCCA350580.19943121884171636Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTG349720.19894199855475225Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACACGTCTGAACT348030.1979806238047879Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACG333890.18993693210981993No Hit
ACCGGTCGGTCACTGGTTCGAGTCCAGTTGGGTCCAGATCGGAAGAGCAC332550.189174658639434No Hit
ATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGA314480.17889534400519982Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGATCGGAAGAGCACA308800.17566421466804158No Hit
GGCTCTGTAGCTCAGTAGGTAGAGCAACGGCAGATCGGAAGAGCACACGT299490.17036812063125573No Hit
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC299280.17024865986350868Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGAACGAAAGTCGGAGGTTCGAAGACGATCAGAGATCGGAAGAGCACACG296030.1683998622674234No Hit
ACCGGTCGGTCACTGGTTCGAGTCCAGTTGGGTAGATCGGAAGAGCACAC287610.16361005434156553No Hit
ACTGAATGTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTA285540.1624325124880589TruSeq Adapter, Index 11 (100% over 38bp)
ATGGGGGTAGAGCACTGAATGTGGAATGGCGGCAAGATCGGAAGAGCACA281210.1599693452292745No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT280510.15957114267011768No Hit
GATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACACGTCTGA271660.15453672460077775Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCTCTGTAGCTCAGTAGGTAGAGCAACGGAGATCGGAAGAGCACACGTC269170.15312026121177702No Hit
TCGCTGCGATCTATTGAAAGAGATCGGAAGAGCACACGTCTGAACTCCAG267600.15222714975766813Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCATGGATTCACGGGTTCGATTCCCGTACGGGTCAGATCGGAAGAGCAC266040.1513397269115472No Hit
GCAAGTGTAGTTTAATGGCAGAACTTCAGCAGATCGGAAGAGCACACGTC265560.15106667372812538No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC262410.14927476221191965No Hit
TCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAA258180.1468684810330148Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT254290.14465561252570042No Hit
TTGGAATGAGTCCACTTTAAATCCTTTAACGAGGAGATCGGAAGAGCACA250440.14246549845033785No Hit
TCGCTGCGATCTATTGAAAGTCAGATCGGAAGAGCACACGTCTGAACTCC243650.1386029336265166Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCAGATCGGAAGAGCACACGTCTGA237080.13486551817843034Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCGGAGTAGAGCAGTTGGAAGCTCGTTGGGAGATCGGAAGAGCACACGT231870.13190175341670596No Hit
ATGGACAACAGGTTAATATTCCTGTACTTGGTAGATCGGAAGAGCACACG229170.13036582925995818No Hit
TCTCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC225740.1284146367200897No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTG222480.12656015051601646Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACA221710.12612212770094394No Hit
AGCAGTAGGTCGTAGGTTCAAATCCTATAGACGGAGATCGGAAGAGCACA216290.1230389021714725No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA209200.11900567910801262No Hit
GGCTCTGTAGCTCAGTAGGTAGAGCAACGGTTAGATCGGAAGAGCACACG209030.11890897277221739No Hit
AGAGGTGTAGTTCAATGGTAGAACAACGGGCTTCAGATCGGAAGAGCACA207600.11809550182993987No Hit
AGAAGATCGCGGGTTCGATTCCCGTCTGGACCGCAGATCGGAAGAGCACA206920.11770867648675894No Hit
ATCCATGGATTCACGGGTTCGATTCCCGTACGGGTCAGATCGGAAGAGCA206500.11746975495126484No Hit
TCGCTGCGATCTATTGAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT206100.11724221063174665Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAAAAGGGGTTGTAGGACATCAGATCGGAAGAGCACACGTCTGAACTCCA205950.11715688151192732Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAGCTATGCAGTCGGAACGTGGGTGAGATCGGAAGAGCACACGTCTGAAC200320.11395419521470884Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACTCCGGATGTGCTGACCCCTGCGATTTCCCCAGATCGGAAGAGCACACG200190.11388024331086541No Hit
GCCGATTTAGCTCAGCGGTAGAGCAGCTGAGATCGGAAGAGCACACGTCT195610.11127486085238215Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTG193620.11014282786277917Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCGTCTTAGCTCAGCTGGCAGAGCAACTGAGATCGGAAGAGCACACGTC193290.10995510379917667No Hit
TGGGGGTAGAGCACTGAATGTGGAATGGCGGCAGATCGGAAGAGCACACG185740.10566020476827084No Hit
TAGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC182370.10374314387633012No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG176890.10062578669893094No Hit
ATCTTGAGTTGTGATGCATAGTGAAGACTTCGGTCGAGATCGGAAGAGCA176290.10028447021965366No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)