FastQCFastQC Report
Fri 30 Jun 2023
SRR2007780.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2007780.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16585777
Total Bases845.8 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA6269353.7799555607192836No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACAC3872372.3347534456781855No Hit
GGCTCTGTAGCTCAGTAGGTAGAGCAACGGTAGATCGGAAGAGCACACGT3037631.8314668043589395No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC2696951.6260618962861975No Hit
ATTGTTTAATGGGGGTAGAGCACTGAATGAGATCGGAAGAGCACACGTCT1991381.2006552361098308Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC1981651.1947887638908927No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA1767801.0658529895825803No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC1764591.0639175963839378No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG1718481.036116667913719No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAGATCGGAAGAGCACAC1684161.0154242396964581No Hit
ATGGGGGTAGAGCACTGAATGTGGAATGGCGGCAGATCGGAAGAGCACAC1596130.9623486436601674No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC1131000.6819095662506496No Hit
TATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACT1099390.6628510681169776Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCTGTACTGACTATAATCAAACTCCGAATACCATTAGATCGGAAGAGCA1068120.6439975649015418No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC1038640.626223299637997No Hit
GGGAGCGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAG937200.5650624628559759TruSeq Adapter, Index 10 (100% over 36bp)
ATTGTTTAATGGGGGTAGAGCACTGAATGTGGAGATCGGAAGAGCACACG860600.5188783136298046No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA801310.4831308174467798No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA798540.4814607117893843No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA727850.43883985658314345No Hit
AGCGGAGTAGAGCAGTTGGAAGCTCGTTGGGCAGATCGGAAGAGCACACG698870.421367054434652No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC621430.37467644717519116No Hit
ATTGTTTAATGGGGGTAGAGCACTGAATAGATCGGAAGAGCACACGTCTG555580.33497375492266657Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCTCTGTAGCTCAGTAGGTAGAGCAACGGTTAGATCGGAAGAGCACACG511980.3086861712900155No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCT509440.3071547386655446Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCGATTTAGCTCAGCGGTAGAGCAGCTGGCAGATCGGAAGAGCACACG505260.30463450702369865No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT444280.2678680655117936No Hit
ATTGTTTAATGGGGGTAGAGCACTGAATGTGAGATCGGAAGAGCACACGT436620.2632496505891765No Hit
AGCCGATTTAGCTCAGCGGTAGAGCAGCTGGAGATCGGAAGAGCACACGT435780.2627431925558869No Hit
AACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACACGT432760.26092235534096475No Hit
ACCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA432630.2608439749310509No Hit
AGAGGTGTAGTTCAATGGTAGAACAACGGGCTTCAGATCGGAAGAGCACA415270.250377175576399No Hit
TTGGAATGAGTCCACTTTAAATCCTTTAACGAGGAGATCGGAAGAGCACA408920.24654859401522158No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG394500.23785439777708334TruSeq Adapter, Index 10 (100% over 49bp)
TATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACACGTCTGAACTC385150.2322170375255859Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGAACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACA376990.22729715948791546No Hit
ACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT359450.21672183341184437Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGCGAGGGTCGTGGGTTCGATCCCCTCATCTTCCAGATCGGAAGAGCAC329900.19890536331219213No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTAGATCGGAAGAGCACACG324050.19537824486606808No Hit
GCAAGTGTAGTTTAATGGCAGAACTTCAGCAGATCGGAAGAGCACACGTC322130.19422062650426325No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA318950.19230332109252404No Hit
AGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGATCGGAAGAGCACA317370.1913506976489555No Hit
GGCTCTGTAGCTCAGTTGGTTAGAGCGTTCGGAGATCGGAAGAGCACACG311810.18799842780956236No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC300270.18104065911413136No Hit
ATTGTTTAATGGGGGTAGAGCACTGAATGTAGATCGGAAGAGCACACGTC294990.1778572086191681No Hit
AGAACGAAAGTCGGAGGTTCGAAGACGATCAGAGATCGGAAGAGCACACG294410.17750751140570623No Hit
ATGGACAACAGGTTAATATTCCTGTACTTGGTAGATCGGAAGAGCACACG293300.17683826329028782No Hit
GCCGATTTAGCTCAGCGGTAGAGCAGCTGGCAGATCGGAAGAGCACACGT292150.17614489812566514No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC282090.170079460250792No Hit
ATCTTGAGTTGTGATGCATAGTGAAGACTTCGGTCGAGATCGGAAGAGCA277270.1671733558216778No Hit
ATGGGGGTAGAGCACTGAATGTGGAATGGCGGCAAGATCGGAAGAGCACA276970.1669924779526458No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC272420.16424916360566044No Hit
TAGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC259290.1563327422043598No Hit
TACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCA258250.15570569892504887Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AACTGGTGCGGACCAGGGGAATCCGACTGTTTAGATCGGAAGAGCACACG256820.1548435144159963No Hit
GGACCTATAGCTCAGTTGGTTAGAGCAACCGGCAGATCGGAAGAGCACAC247320.1491157152299829No Hit
GCCTCTGTAGCTCAGTAGGTAGAGCAACGGTAGATCGGAAGAGCACACGT245600.14807868211419942No Hit
GGCTCTGTAGCTCAGTAGGTAGAGCAACGGCAGATCGGAAGAGCACACGT236920.14284528243687347No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAAGATCGGAAGAGCACAC233480.14077121620530653No Hit
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACAC227590.13721998070997818No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG219390.13227598562310347No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATCGGAAGAGCACACGTCTG215190.12974369545665543Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC209370.1262346647974346Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCGTCTTAGCTCAGCTGGCAGAGCAACTGCAGATCGGAAGAGCACACGT204510.12330444331911614No Hit
TAGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC192870.11628638200067443No Hit
GGTCGCGTAGCTCAGTTGGATAGAGCACGAGCAGATCGGAAGAGCACACG192060.11579801175428803No Hit
TCCTCGCGTCGGGGTCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCA187660.11314513634181865Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTG185590.11189707904549784Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAACTGGTGCGGACCAGGGGAATCCGACTGTCAGATCGGAAGAGCACAC174200.10502974928458281No Hit
AGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGTC168220.1014242504285449No Hit
AAAAAGGGGTTGTAGGACATCAGATCGGAAGAGCACACGTCTGAACTCCA167020.10070073895241688Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)