FastQCFastQC Report
Fri 30 Jun 2023
SRR2007781.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2007781.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20561299
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC351170217.079183567147194No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG14023606.8203862022530775No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA4576962.2260072187073394No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC2496261.2140575359562642No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA2365941.1506763264324884No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA1848820.899174706812055No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC1797150.8740449715749964No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA1447350.7039195334886186No Hit
TATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACT1332940.6482761619292633Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCA1028920.5004158540761457No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACA911960.4435322884998657No Hit
TCTCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC904030.4396755282825273No Hit
TCCTCCCGACCCCTCCACCCGCGCGTCGTTCCCCAGATCGGAAGAGCACA876270.4261744357688685No Hit
TCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTG811200.39452760256051916Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAAC772270.3755939739021353Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG768100.37356589192151723Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTG721280.35079495706958985Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC711760.3461648994064042No Hit
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC664880.32336478351878445Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC619920.3014984607733198No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT619880.30147900674952494No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAGATCGGAAGAGCACACG617260.30020476819095915No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC591890.28786605359904543No Hit
GTTTCCGTAGTGTAGTGGTCATCACGCTCGCCTGAGATCGGAAGAGCACA551790.26836339474466087No Hit
AGCCGATTTAGCTCAGCGGTAGAGCAGCTGGCAGATCGGAAGAGCACACG526880.25624840142638844No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA525480.2555675105935671No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC487850.23726613770851732No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG440560.2142666180769999No Hit
GACCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG429220.20875140233114647No Hit
GTTTCTGTAGTGTAGTGGTTATCACGCTCGCCTGAGATCGGAAGAGCACA406820.19785714900600396No Hit
GCCGATTTAGCTCAGCGGTAGAGCAGCTGGCTGAGATCGGAAGAGCACAC402790.19589715610866804No Hit
TGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGT379620.18462841282547374No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACGAGATCGGAAGAGCAC361710.1759178736713084No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG338620.1646880384357039No Hit
TCCTCCCGACCCCTCCACCCGCGCGTCGTTCCCCTCAGATCGGAAGAGCA334140.16250918777067538No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGAGATCGGAAGAGCACA316800.15407586845558738No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC300930.14635748451496183No Hit
AGCTGTACTGACTATAATCAAACTCCGAATACCATTAGATCGGAAGAGCA295680.14380414389188154No Hit
AGCGGAGTAGAGCAGTTGGAAGCTCGTTGGGCAGATCGGAAGAGCACACG293120.1425590863690081No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAGATCGGAAGAGCACA286540.13935889945474747No Hit
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGATCGGAAGAGCACAC278820.1356042728623323No Hit
CCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC277620.13502065214848535No Hit
ATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGA265110.12893640620663122Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACG260630.1267575555416027No Hit
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC248430.12082407828415899No Hit
TCGCTGCGATCTATTGAAAGTAGATCGGAAGAGCACACGTCTGAACTCCA244720.11901971757718224Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTCTGTAGCTCAGTAGGTAGAGCAACGGTAGATCGGAAGAGCACACGT242200.11779411407810372No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAGATCGGAAGAGCACA236330.11493923608620253No Hit
TCCTCCCGACCCCTCCACCCGCGCGTCGTTCCCCTAGATCGGAAGAGCAC235900.1147301053304074No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC234260.11393249035481659No Hit
GCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC233870.11374281362281632No Hit
TCGATGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC233310.11347045728968777No Hit
TCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAA223420.1086604499063994Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC211590.1029069223690585No Hit
TCGCTGCGATCTATTGAAAGTCAGATCGGAAGAGCACACGTCTGAACTCC206860.10060648405531188Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC205620.10000340931767006TruSeq Adapter, Index 6 (100% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)