FastQCFastQC Report
Fri 30 Jun 2023
SRR2007782.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2007782.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15876938
Total Bases809.7 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG166592210.49271591285423No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC4056242.5547999242675132No Hit
GACCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG2220021.3982670965900352No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC1803611.1359936027967106No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA1708791.0762717597058074No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA1673021.053742226618256No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC1517510.9557951287584545No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA1507940.9497675181448715No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC1493240.9405088059171108No Hit
TATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACT1317250.8296624953753677Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA895570.5640697217561724No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG852150.5367218792439701No Hit
ACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG702400.4424026849509648Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC669830.4218886538449668No Hit
TCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG643980.405607176900231No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAGATCGGAAGAGCACAC623310.39258829378813476No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC623060.39243083269582585No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACAC594790.3746251323775403No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAGATCGGAAGAGCACACG510240.32137179095868484No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT491220.309392151055827No Hit
GGCTCTGTAGCTCAGTAGGTAGAGCAACGGTAGATCGGAAGAGCACACGT474470.2988422578711336No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA397040.2500734083612344No Hit
TAGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC375570.23655064975374976No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAGATCGGAAGAGCACA370360.23326916059003316No Hit
TCCTCCCGACCCCTCCACCCGCGCGTCGTTCCCCAGATCGGAAGAGCACA360200.2268699417986012No Hit
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC340390.2143927248440474Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG328700.20702984416768522TruSeq Adapter, Index 6 (100% over 49bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT320320.20175174835349233No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATAGATCGGAAGAGCACACG302520.19054051858110171No Hit
TATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACACGTCTGAACTC300170.18906038431339847Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG290570.18301387836873834No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT281430.17725710083392654No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATCGGAAGAGCACACGTCTG269980.1700453828061809Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTTCTGTAGTGTAGTGGTTATCACGCTCGCCTGAGATCGGAAGAGCACA267970.16877939562401767No Hit
AGCGGAGTAGAGCAGTTGGAAGCTCGTTGGGCAGATCGGAAGAGCACACG264690.16671350609292548No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGGCAGATCGGAAGAGCACACG234750.14785596567801676No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACAC220300.1387547145425648No Hit
TCTCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC218650.13771547133332634No Hit
AGCTGTACTGACTATAATCAAACTCCGAATACCATTAGATCGGAAGAGCA213810.13466702458622687No Hit
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACAC208460.13129735721081737No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACACG200100.12603185828400917No Hit
GCCCGACTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG196210.12358176368768335No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC190140.11975860836642431No Hit
GCGCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG186630.11754785463040797No Hit
TCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTG185220.11665977406978599Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC184560.11624407678609062No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG184050.11592285615778056No Hit
CCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC178440.11238942924636981TruSeq Adapter, Index 6 (100% over 35bp)
GTCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG170130.10715542253802339No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG164360.10352122052753497No Hit
AACTGGTGCGGACCAGGGGAATCCGACTGTTTAGATCGGAAGAGCACACG162890.10259534930475889No Hit
TCCTCCCGACCCCTCCACCCGCGCGTCGTTCCCCTCAGATCGGAAGAGCA162530.10236860533183413No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)