FastQCFastQC Report
Fri 30 Jun 2023
SRR2007783.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2007783.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8558520
Total Bases436.4 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGTTTAATGGGGGTAGAGCACTGAATGAGATCGGAAGAGCACACGTCT1827662.1354860419792208Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC1768022.065801096451256No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC1644101.9210097072858392No Hit
GGCTCTGTAGCTCAGTAGGTAGAGCAACGGTAGATCGGAAGAGCACACGT1432481.6737473301458663No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA1223051.4290438066394655No Hit
TATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACT886081.035319190701196Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA880511.028811056117179No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC743180.8683510700448208No Hit
AGCTGTACTGACTATAATCAAACTCCGAATACCATTAGATCGGAAGAGCA699520.8173375770577156No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA665900.7780550842902745No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC630590.736797951047611No Hit
ATGGGGGTAGAGCACTGAATGTGGAATGGCGGCAGATCGGAAGAGCACAC581960.6799773792665087No Hit
ATTGTTTAATGGGGGTAGAGCACTGAATAGATCGGAAGAGCACACGTCTG569470.6653837345709305Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA446680.5219126671433846No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACAC405620.47393708257969835No Hit
AGCCGATTTAGCTCAGCGGTAGAGCAGCTGGAGATCGGAAGAGCACACGT389550.4551604716703355No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC316360.3696433495510906No Hit
TATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACACGTCTGAACTC290200.3390773171062286Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCGATTTAGCTCAGCGGTAGAGCAGCTGGCAGATCGGAAGAGCACACGT280080.3272528427812285No Hit
ATTGTTTAATGGGGGTAGAGCACTGAATGTGGAGATCGGAAGAGCACACG269250.3145987857713717No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA238300.27843599126951857No Hit
GGCTCTGTAGCTCAGTAGGTAGAGCAACGGCAGATCGGAAGAGCACACGT236670.2765314563732982No Hit
ATTGTTTAATGGGGGTAGAGCACTGAATGTGAGATCGGAAGAGCACACGT233120.27238354294901457No Hit
TTGGAATGAGTCCACTTTAAATCCTTTAACGAGGAGATCGGAAGAGCACA228050.2664596215233475No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG227680.2660273037861686No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCT215530.25183092403826834Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTGTTTAATGGGGGTAGAGCACTGAATGTAGATCGGAAGAGCACACGTC214700.2508611301954076No Hit
TGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGT200610.23439800339311004No Hit
AGCCGATTTAGCTCAGCGGTAGAGCAGCTGGCAGATCGGAAGAGCACACG196910.23007482602132143No Hit
GATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTG173560.2027920715263854Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTGTTTAATGGGGGTAGAGCACTAGATCGGAAGAGCACACGTCTGAACT168310.1966578333637124Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC162490.18985759220052065No Hit
TCGCCCGTCACGTTGAACGCACGTTAGATCGGAAGAGCACACGTCTGAAC152030.17763585292784267Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATGGACAACAGGTTAATATTCCTGTACTTGGTAGATCGGAAGAGCACACG148710.17375667755639992No Hit
GCAAGTGTAGTTTAATGGCAGAACTTCAGCAGATCGGAAGAGCACACGTC147350.17216761776568845No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC146440.1711043498174918No Hit
TACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCA137480.16063525001986323Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAAC127840.14937162032687895Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGCTGCGATCTATTGAAAGTAGATCGGAAGAGCACACGTCTGAACTCCA126360.1476423493781635Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT125850.1470464519566467No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT125560.1467076083248038No Hit
AGCGGAGTAGAGCAGTTGGAAGCTCGTTGGGCAGATCGGAAGAGCACACG125270.14636876469296092No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACGAGATCGGAAGAGCAC116540.13616840294817328No Hit
GGCTCTGTAGCTCAGTAGGTAGAGCAACGGAGATCGGAAGAGCACACGTC115770.13526871468431456No Hit
TAGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA113070.13211396362922562No Hit
GGGAGCGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC110770.1294265831008165TruSeq Adapter, Index 1 (100% over 36bp)
TTTGTTTAATGGGGGTAGAGCACTGAATGAGATCGGAAGAGCACACGTCT110260.1288306856792997Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTCTGTAGCTCAGTTGGTTAGAGCGTTCGGAGATCGGAAGAGCACACG109770.12825815678411687No Hit
AGAACGAAAGTCGGAGGTTCGAAGACGATCAGAGATCGGAAGAGCACACG107420.12551235493987278No Hit
ACCGGTCGGTCACTGGTTCGAGTCCAGTTGGGTAGATCGGAAGAGCACAC104370.12194865467393895No Hit
GGCTCTGTAGCTCAGTAGGTAGAGCAACGGGAGATCGGAAGAGCACACGT103500.1209321237784103No Hit
ACCGGTCGGTCACTGGTTCGAGTCCAGTTGGGTCCAGATCGGAAGAGCAC102780.12009085683038656No Hit
ATCTTGAGTTGTGATGCATAGTGAAGACTTCGGTCGAGATCGGAAGAGCA95560.11165481882381534No Hit
ACCGGTCGGTCACTGGTTCGAGTCCAGTTGGGTCAGATCGGAAGAGCACA93340.1090609124007422No Hit
ATGGGGGTAGAGCACTGAATGTGGAATGGCGGCAAGATCGGAAGAGCACA91520.10693437650434888No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC87210.10189845907937355No Hit
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC86650.10124414034202175Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCGATTTAGCTCAGCGGTAGAGCAGCTGGCTGAGATCGGAAGAGCACAC85910.10037950486766405No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)