FastQCFastQC Report
Fri 30 Jun 2023
SRR2007784.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2007784.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35387962
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA12120973.4251675753466673No Hit
ATTGTTTAATGGGGGTAGAGCACTGAATGAGATCGGAAGAGCACACGTCT10875883.073327590890936Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTCTGTAGCTCAGTAGGTAGAGCAACGGTAGATCGGAAGAGCACACGT8312532.3489710992681636No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC5179511.4636361370570026No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC4355661.230830981450698No Hit
ATGGGGGTAGAGCACTGAATGTGGAATGGCGGCAGATCGGAAGAGCACAC4079821.1528835709725245No Hit
AGCTGTACTGACTATAATCAAACTCCGAATACCATTAGATCGGAAGAGCA3758081.0619656480924218No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA3537560.9996506721692534No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAGATCGGAAGAGCACAC3149060.8898675770025977No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACAC2834860.8010803221728338No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC2748920.7767952277104853No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC2582750.729838581831867No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA2395020.676789468689946No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA2220880.6275806445140865No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG2217230.6265492203252621No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC2044000.5775975457416846No Hit
ATTGTTTAATGGGGGTAGAGCACTGAATAGATCGGAAGAGCACACGTCTG1932230.5460133590060936Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTGTTTAATGGGGGTAGAGCACTGAATGTGGAGATCGGAAGAGCACACG1792000.5063868894173674No Hit
GGGAGCGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGC1732330.4895252232948595TruSeq Adapter, Index 11 (100% over 36bp)
ATTGTTTAATGGGGGTAGAGCACTGAATGTGAGATCGGAAGAGCACACGT1660620.4692612702590785No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT1415770.4000710750169789No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCT1389320.39259678192262104Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCGATTTAGCTCAGCGGTAGAGCAGCTGGAGATCGGAAGAGCACACGT1310480.3703180194440132No Hit
GCAAGTGTAGTTTAATGGCAGAACTTCAGCAGATCGGAAGAGCACACGTC1285870.3633636771736106No Hit
GCCGATTTAGCTCAGCGGTAGAGCAGCTGGCAGATCGGAAGAGCACACGT1115280.31515801898962137No Hit
AGCGGAGTAGAGCAGTTGGAAGCTCGTTGGGCAGATCGGAAGAGCACACG1050030.29671954547707496No Hit
TATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACT1038240.29338790405618725Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTGGAATGAGTCCACTTTAAATCCTTTAACGAGGAGATCGGAAGAGCACA935100.2642423997177345No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC921930.2605207951788803No Hit
ATGGGGGTAGAGCACTGAATGTGGAATGGCGGCAAGATCGGAAGAGCACA897680.2536681824175125No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG871300.24621367006102246TruSeq Adapter, Index 11 (100% over 49bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC822320.23237280519290715No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA821180.23205066174763045No Hit
AGAGGTGTAGTTCAATGGTAGAACAACGGGCTTCAGATCGGAAGAGCACA790650.22342343421754549No Hit
ATTGTTTAATGGGGGTAGAGCACTGAATGTAGATCGGAAGAGCACACGTC736360.20808205909116778No Hit
GGCTCTGTAGCTCAGTAGGTAGAGCAACGGCAGATCGGAAGAGCACACGT724120.20462325578398666No Hit
TTTGTTTAATGGGGGTAGAGCACTGAATGAGATCGGAAGAGCACACGTCT711310.2010033807541672Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCGATTTAGCTCAGCGGTAGAGCAGCTGGCAGATCGGAAGAGCACACG710300.20071797296493085No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA611650.17284126166971697No Hit
AAAAAGGGGTTGTAGGACATCAGATCGGAAGAGCACACGTCTGAACTCCA590670.166912691948748Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG590620.16689856285027094Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC585380.16541783332987642Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC574030.16221052797558674No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC573900.1621737923195464No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATCGGAAGAGCACACGTCTG561400.15864151770028462Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCTCTGTAGCTCAGTAGGTAGAGCAACGGAGATCGGAAGAGCACACGTC538550.1521845196962741No Hit
GGCTCTGTAGCTCAGTAGGTAGAGCAACGGTTAGATCGGAAGAGCACACG523120.1478242799062574No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAAGATCGGAAGAGCACAC521700.14742301350950923No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC501470.141706380265696No Hit
ACTGTTTAATGGGGGTAGAGCACTGAATGAGATCGGAAGAGCACACGTCT500210.1413503269840744Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTG494770.1398130810697717Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC491160.1387929601597289No Hit
AGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGATCGGAAGAGCACA486670.13752416711649007No Hit
GGCTCTGTAGCTCAGTTGGTTAGAGCGTTCGGAGATCGGAAGAGCACACG483110.13651817530492433No Hit
ACTCCGGATGTGCTGACCCCTGCGATTTCCCCAGATCGGAAGAGCACACG470540.13296611994779467No Hit
GGTCGCGTAGCTCAGTTGGATAGAGCACGAGCAGATCGGAAGAGCACACG463860.1310784723912612No Hit
AGAACGAAAGTCGGAGGTTCGAAGACGATCAGAGATCGGAAGAGCACACG462720.13075632894598452No Hit
ATGGACAACAGGTTAATATTCCTGTACTTGGTAGATCGGAAGAGCACACG457320.12923038631046344No Hit
ATCTTGAGTTGTGATGCATAGTGAAGACTTCGGTCGAGATCGGAAGAGCA430740.1217193575600652No Hit
ACGGGACTCAAACACAATACCGAAGCTACGGGCACAAGATCGGAAGAGCA422280.11932871409774883No Hit
TAGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC416690.11774908088801497No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG397650.11236872018795545No Hit
AGGCGAGGGTCGTGGGTTCGATCCCCTCATCTTCCAGATCGGAAGAGCAC397420.11230372633496102No Hit
TAGAACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACA387310.10944682262290212No Hit
TGCTCTGTAGCTCAGTAGGTAGAGCAACGGTAGATCGGAAGAGCACACGT365760.10335718117929481No Hit
GCCGATTTAGCTCAGCGGTAGAGCAGCTGGCTGAGATCGGAAGAGCACAC362370.10239922830255102No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG356590.10076590451860439No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)