FastQCFastQC Report
Fri 30 Jun 2023
SRR2007785.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2007785.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11957757
Total Bases609.8 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC3685263.081899055148888No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG1913231.5999907005971103No Hit
GGCTCTGTAGCTCAGTAGGTAGAGCAACGGTAGATCGGAAGAGCACACGT1624991.3589421494348815No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC1467711.2274124653979839No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA1396841.1681454975209817No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA1395211.1667823656225829No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG952670.7966962365935351TruSeq Adapter, Index 1 (100% over 49bp)
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC870590.7280546008753983No Hit
AGCTGTACTGACTATAATCAAACTCCGAATACCATTAGATCGGAAGAGCA827610.692111405174064No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACAC814930.6815074097926559No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA784370.6559507773907766No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA656550.5490578207936488No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA541840.45312845879039015No Hit
ATGGGGGTAGAGCACTGAATGTGGAATGGCGGCAGATCGGAAGAGCACAC537910.4498418892439443No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC451440.37752899644975224No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC446450.3733559730307281No Hit
AGCCGATTTAGCTCAGCGGTAGAGCAGCTGGAGATCGGAAGAGCACACGT444170.3714492609274465No Hit
TATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACT353290.29544838551243346Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCGATTTAGCTCAGCGGTAGAGCAGCTGGCAGATCGGAAGAGCACACGT312850.2616293339963339No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACGAGATCGGAAGAGCAC296340.24782239679230814No Hit
GGCTCTGTAGCTCAGTAGGTAGAGCAACGGCAGATCGGAAGAGCACACGT266180.2226002752857413No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACAC247910.20732149014234025No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCT244410.20439451980835535Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCGATTTAGCTCAGCGGTAGAGCAGCTGGCAGATCGGAAGAGCACACG238680.19960265123300297No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT229330.19178345905507194No Hit
GATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTG213100.17821067947776495Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAAC196360.1642113985089344Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC193810.16207889155131686No Hit
TCGCTGCGATCTATTGAAAGTAGATCGGAAGAGCACACGTCTGAACTCCA192390.1608913778729573Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC185670.15527159483170633Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCGGAGTAGAGCAGTTGGAAGCTCGTTGGGCAGATCGGAAGAGCACACG174510.14593874085248598No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG173190.14483485489795453No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC169560.14179916852299307No Hit
ATGGACAACAGGTTAATATTCCTGTACTTGGTAGATCGGAAGAGCACACG169040.14136430435908676No Hit
GCAAGTGTAGTTTAATGGCAGAACTTCAGCAGATCGGAAGAGCACACGTC168310.14075382197514133No Hit
GGGAGCGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC162910.13623792488842182TruSeq Adapter, Index 1 (100% over 36bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATAGATCGGAAGAGCACACG161020.13465736090806998No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT155110.12971496242982694No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC149900.12535795801838087No Hit
TACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCA146290.12233899718818504Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGCCCGTCACGTTGAACGCACGTTAGATCGGAAGAGCACACGTCTGAAC145420.12161143599088023Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGT143190.11974653774951272No Hit
AGAACGAAAGTCGGAGGTTCGAAGACGATCAGAGATCGGAAGAGCACACG140360.11737987316517637No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAGATCGGAAGAGCACA140090.1171540783108404No Hit
ACCGGTCGGTCACTGGTTCGAGTCCAGTTGGGTAGATCGGAAGAGCACAC138950.11620072225919961No Hit
ACCGGTCGGTCACTGGTTCGAGTCCAGTTGGGTCCAGATCGGAAGAGCAC133710.1118186295306051No Hit
GGCTCTGTAGCTCAGTAGGTAGAGCAACGGAGATCGGAAGAGCACACGTC130440.10908400296142495No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTCAGATCGGAAGAGCACAC129140.10799684255165916No Hit
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC129120.10798011700689351Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCGATTTAGCTCAGCGGTAGAGCAGCTGGCTGAGATCGGAAGAGCACAC125920.10530402984439305No Hit
GGCTCTGTAGCTCAGTTGGTTAGAGCGTTCGGAGATCGGAAGAGCACACG125320.10480226350142421No Hit
AGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGATCGGAAGAGCACA121470.10158259613404085No Hit
ACCGGTCGGTCACTGGTTCGAGTCCAGTTGGGTCAGATCGGAAGAGCACA120750.10098047652247827No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)