FastQCFastQC Report
Fri 30 Jun 2023
SRR2007787.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2007787.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20017242
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC11019655.505079071332604No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC7606523.799984033764492No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA6973303.4836467481384297No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC5544772.7699969855987154No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA5303002.649216110790887No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA3978061.9873167342434088No Hit
AGCTGTACTGACTATAATCAAACTCCGAATACCATTAGATCGGAAGAGCA2511371.2546034064033396No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA1798480.8984654329502536No Hit
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACAC1530060.764371035730097No Hit
ATGGGGGTAGAGCACTGAATGTGGAATGGCGGCAGATCGGAAGAGCACAC998520.4988299586926111No Hit
ACCGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCA972820.48599102713550646No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT740900.37013091014236627No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC705910.352650979590495No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAACAGATCGGAAGAGCAC661120.33027526969000026No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG603070.301275270589225No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA531550.26554607273069886No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACTAGATCGGAAGAGCA511580.25556967338457515No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCA504110.2518378905545529No Hit
TATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACT448690.22415175876876545Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAGATCGGAAGAGCACAC387850.1937579612615964No Hit
CTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAAC370620.1851503818558021Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AACGGAGAGAGGTGGTATCCCCGGTGGCGAGATCGGAAGAGCACACGTCT355030.17736209613692036Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTG344020.17186183790953818Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC323440.1615807012774287No Hit
GGCTCTGTAGCTCAGTAGGTAGAGCAACGGTAGATCGGAAGAGCACACGT301470.1506051632887288No Hit
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC280870.14031403527019357Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGCTGCGATCTATTGAAAGTAGATCGGAAGAGCACACGTCTGAACTCCA278300.1390301421144831Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATGGGGGTAGAGCACTGAATGTGGAATGGCGGCAAGATCGGAAGAGCACA264560.13216605963998437No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA243210.12150025463048306No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCAC229590.11469612047453889No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC227650.11372695599123994No Hit
GGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCAC211230.10552402773568906No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAAGATCGGAAGAGCACAC210820.10531920431396094No Hit
AGGAATAATGGAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA209350.10458483741166741TruSeq Adapter, Index 7 (100% over 35bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACATAGATCGGAAGAGCACA207790.10380550927045794No Hit
TCTCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC207170.1034957762912593No Hit
TTGGAATGAGTCCACTTTAAATCCTTTAACGAGGAGATCGGAAGAGCACA201580.10070318378525872No Hit
TGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGT201060.10044340773818891No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)