FastQCFastQC Report
Fri 30 Jun 2023
SRR2007788.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2007788.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14374170
Total Bases733 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA2581801.7961384900832533No Hit
GGCTCTGTAGCTCAGTAGGTAGAGCAACGGTAGATCGGAAGAGCACACGT1862911.2960122219230745No Hit
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC1632071.1354186015609944Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC1513741.0530973266630352No Hit
AGCTGTACTGACTATAATCAAACTCCGAATACCATTAGATCGGAAGAGCA1221560.8498299380068554No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA910690.6335600594677814No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC855990.5955056883284391No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG820420.5707599117027279TruSeq Adapter, Index 8 (100% over 49bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC739910.5147497212012937No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA707720.49235538469351625No Hit
GCCGATTTAGCTCAGCGGTAGAGCAGCTGGCAGATCGGAAGAGCACACGT491180.34171016483038674No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG477540.3322209212775416No Hit
AGCCGATTTAGCTCAGCGGTAGAGCAGCTGGCAGATCGGAAGAGCACACG472890.3289859518845262No Hit
ATGGACAACAGGTTAATATTCCTGTACTTGGTAGATCGGAAGAGCACACG406250.28262501417473146No Hit
ACTCCGGATGTGCTGACCCCTGCGATTTCCCCAGATCGGAAGAGCACACG375340.26112116386546147No Hit
CTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACACGT370590.25781662523818766No Hit
GGCTCTGTAGCTCAGTAGGTAGAGCAACGGCAGATCGGAAGAGCACACGT352060.244925446130107No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC351880.24480022150844188No Hit
TCCCGTGGATCGCCTCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGT341820.2378015565420473Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCTCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA324260.22558519900627308No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACATAGATCGGAAGAGCACA322610.22443730664100953No Hit
ACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA320350.2228650419467698Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTGGAATGAGTCCACTTTAAATCCTTTAACGAGGAGATCGGAAGAGCACA320020.2226354634737171No Hit
ACCGGAGTGGACGTACCGCTGGTGTTCCAGTAGATCGGAAGAGCACACGT305890.2128053306730058No Hit
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT300240.20887466893740647Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACAGATCGGAAGAGCACACG299850.20860334892379875No Hit
TCGTTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA291850.2030378101831271No Hit
GGGAGCGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACT290540.20212645321434208TruSeq Adapter, Index 8 (100% over 36bp)
ATGGGGGTAGAGCACTGAATGTGGAATGGCGGCAGATCGGAAGAGCACAC285970.19894713920873344No Hit
GCCGATTTAGCTCAGCGGTAGAGCAGCTGGCTAGATCGGAAGAGCACACG280160.19490516669832067No Hit
AGCCGATTTAGCTCAGCGGTAGAGCAGCTGGAGATCGGAAGAGCACACGT263240.18313405226180018No Hit
CTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACG262820.1828418614779149No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT259750.18070608598618215No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACAC247000.17183600861823675No Hit
GCCGATTTAGCTCAGCGGTAGAGCAGCTGGCTGAGATCGGAAGAGCACAC244600.17016634699603525No Hit
CTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACACGTCTGAACT233900.16272243893038693Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA230380.1602736018844914No Hit
TCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACT224850.15642642323000217TruSeq Adapter, Index 8 (100% over 36bp)
CTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTT223950.1558003001216766TruSeq Adapter, Index 8 (100% over 37bp)
TCTCGGCGCCCCCTCGATGCTTAGATCGGAAGAGCACACGTCTGAACTCC220720.1535532138551304Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGCTATGCAGTCGGAACGTGGGTGAGATCGGAAGAGCACACGTCTGAAC214840.14946254288073677Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC196430.13665484685376617TruSeq Adapter, Index 5 (100% over 35bp)
CCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA195220.13581305911923958Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA192550.13395556056454042Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACACGTCTGA186710.12989271728385013Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA178100.12390280621420227No Hit
TACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCA173110.12043130142470836Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAGATCGGAAGAGCACAC173100.1204243445012825No Hit
ATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACACGTCTGAA172850.12025042141563652Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCTTGAGTTGTGATGCATAGTGAAGACTTCGGTCGAGATCGGAAGAGCA172710.12015302448767477No Hit
TGGACAACAGGTTAATATTCCTGTACTTGGTAGATCGGAAGAGCACACGT165480.11512316885079277No Hit
AGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACAC163840.1139822334089551No Hit
CAGCCCTCGACACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACT163620.11382918109358663TruSeq Adapter, Index 8 (100% over 36bp)
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA158290.11012114090761414No Hit
TCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCCAG157510.10957850088039865Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGTTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC150500.10470169755888514No Hit
CTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC150150.10445820523898076Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCGGATGTGCTGACCCCTGCGATTTCCCCAGATCGGAAGAGCACACGTC149850.10424949753620556No Hit
AACTAAGTGGAGGACCGAACCGTAGTACGCTAGATCGGAAGAGCACACGT149400.10393643598204279No Hit
TCTCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC146060.10161282355781238No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT144320.1004023188817163No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)