FastQCFastQC Report
Sat 23 Sept 2023
SRR20106154_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR20106154_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207768316
Total Bases7.3 Gbp
Sequences flagged as poor quality0
Sequence length35-101
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNNN158624197.634666972032445No Hit
ATGTACACGGAGTCGACCCAACGCGACNNNNNNNN123391305.938889161521625No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN88795624.27378060858904No Hit
GAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNNN73048773.51587630907111No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACNNNNN68337413.2891160363450216No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN65302533.14304564128055No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNN64427823.100945381874299No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNN47364372.2796724212752437No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNN42362832.03894563018935No Hit
CACTCGCCGAATCCCGGGGCCGAGGGNNNNNNNNN31198191.5015855449297668No Hit
GCCGCGACCGGCTCCGGGACGGCTGGGNNNNNNNN29466531.41823982440133No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN27788881.3374936340149188No Hit
CACTCGCCGAATCCCGGGGCCGAGGGANNNNNNNN23156901.1145539630787593No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNNN21822421.0503247280494876No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNN21573351.0383368559429438No Hit
GAGGGCGCACCACCGGCCCGTCTCGNNNNNNNNNN18783640.9040666239023663No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN17406590.8377884720401738No Hit
ACTCGCCGAATCCCGGGGCCGAGGGANNNNNNNNN12497200.6014969096635504No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN11866530.5711424257777592No Hit
GGGAGAGCGACGCTCAGACAGGNNNNNNNNNNNNN11673990.5618753727589533No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN11052360.5319559889006368No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN10887910.5240409225822479No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN10599520.5101605578783244No Hit
ACGGAGTCGAGCTCAACCCGCAACNNNNNNNNNNN10524180.5065344034458074No Hit
ATGTACACGGAGTCGACCCAACGCGNNNNNNNNNN10208140.49132322947643275No Hit
CTCTAGCATCACCAGTATTAGAGGNNNNNNNNNNN10080560.48518273594709216No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN8413840.40496261229744No Hit
TGTACACGGAGTCGACCCAACGCGACNNNNNNNNN7910980.38075969196381226No Hit
TCTAGCATCACCAGTATTAGAGGNNNNNNNNNNNN7669370.3691308736410031No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNNN7258670.34936366332198604No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATNNNN6972100.3355708961899657No Hit
AGGGCGCACCACCGGCCCGTCTCGCNNNNNNNNNN6636510.3194187702806428No Hit
TGTACACGGAGTCGAGCTCAACCCGCAACNNNNNN6633250.31926186473976137No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN6578790.3166406758574296No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCNNNNNNN6407640.30840313496115546No Hit
CCTCTAGCATCACCAGTATTAGAGGNNNNNNNNNN6405180.3082847338474842No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGANNNNNN6400460.30805755772694426No Hit
ACTCGCCGAATCCCGGGGCCGAGGGNNNNNNNNNN6066670.29199206677884415No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGNNNNNNN5725170.27555548941350616No Hit
CGAGGGCGCACCACCGGCCCGTCTCGNNNNNNNNN5708950.27477481215182015No Hit
GGGAGAGCGACGCTCAGACAGGCNNNNNNNNNNNN5094060.24517982809274924No Hit
CCGCGACCGGCTCCGGGACGGCTGGGNNNNNNNNN5020440.24163645817873403No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGNNNNNNNN5001130.24070705756694877No Hit
CGCGACCGGCTCCGGGACGGCTGGGNNNNNNNNNN4236970.20392762869580172No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN4212850.2027667202154153No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATNNN3963610.19077066591808928No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGNNNNN3898640.18764362512328397No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN3541370.1704480292365656No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGAAGNNNN3502340.1685694944940498No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNNN3431290.16514982005244727No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN3382270.16279046127514457No Hit
GCATGCTCATAAGGAAAGGTTAAAANNNNNNNNNN3194470.15375154698755897No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGNNNNNN3188790.15347816555436683No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNNN3115510.14995116002191597No Hit
CCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN2838740.13663007212322018No Hit
AACGATGCCGACCGGCGATGCGGCGGCGNNNNNNN2759440.13281332077601282No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN2738940.13182664482875242No Hit
CGAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNN2608100.12552924575853036No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN2595930.12494349715959578No Hit
ATGTACACGGAGTCGACCCAACGNNNNNNNNNNNN2551900.12282430974701647No Hit
CTAAACCATTCGTAGACGACCTGCTTNNNNNNNNN2492190.119950435561118No Hit
CTAGCATCACCAGTATTAGAGGNNNNNNNNNNNNN2464640.11862443934906802No Hit
CGGAGTCGAGCTCAACCCGCAACNNNNNNNNNNNN2421580.11655193855448104No Hit
ACACGGAGTCGACCCAACGCGACNNNNNNNNNNNN2404060.1157086915985785No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTNNNN2358520.11351682707964No Hit
GGAGTCGAGCTCAACCCGCAACNNNNNNNNNNNNN2323800.11184573493871895No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNNN2321110.11171626380222478No Hit
CACGGAGTCGAGCTCAACCCGCAACNNNNNNNNNN2292830.11035513230034555No Hit
ACACGGAGTCGAGCTCAACCCGCAACNNNNNNNNN2276120.10955087107699327No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNN2219130.10680791194360933No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGANNNNN2184070.10512045542112398No Hit
CACTCGCCGAATCCCGGGGCCGAGGNNNNNNNNNN2130500.10254210271406348No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)