FastQCFastQC Report
Sat 23 Sept 2023
SRR20106155_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR20106155_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences178403458
Total Bases6.2 Gbp
Sequences flagged as poor quality0
Sequence length35-101
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNNN113004046.334184396806927No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN79707024.4677956858885555No Hit
GAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNNN68661613.84867035480893No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNN65607973.6775055111319648No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNN41852312.3459360300067726No Hit
GAGGGCGCACCACCGGCCCGTCTCGNNNNNNNNNN40862952.290479705836195No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNN40253302.2563071619385315No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN38714382.170046502125536No Hit
CACTCGCCGAATCCCGGGGCCGAGGGNNNNNNNNN30626251.7166847741258466No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN30555621.7127257701473477No Hit
CTCTAGCATCACCAGTATTAGAGGNNNNNNNNNNN28434071.5938071110706835No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN23063801.292788842691603No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNNN22966351.2873265046241424No Hit
CCTCTAGCATCACCAGTATTAGAGGNNNNNNNNNN22254681.2474354617050079No Hit
GCCGCGACCGGCTCCGGGACGGCTGGGNNNNNNNN21005111.1773936579188953No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN19746971.106871482278107No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNN17361150.9731397695217321No Hit
TCTAGCATCACCAGTATTAGAGGNNNNNNNNNNNN15543850.8712751520769288No Hit
CACTCGCCGAATCCCGGGGCCGAGGGANNNNNNNN15426570.8647012884694197No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN13930370.7808352010755307No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN12539810.7028905235682147No Hit
GGGAGAGCGACGCTCAGACAGGNNNNNNNNNNNNN11995240.6723658910243768No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGNNNNNNNN10225290.5731553701162003No Hit
CGAGGGCGCACCACCGGCCCGTCTCGNNNNNNNNN10169860.5700483675602297No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATNNNN10031950.5623181362325387No Hit
ACTCGCCGAATCCCGGGGCCGAGGGANNNNNNNNN9570820.5364705430765809No Hit
TTGATCATCGACACTTCGAACGNNNNNNNNNNNNN8980860.5034016773374427No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCNNNNNNN8831370.49502235545232537No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN7584580.4251363782421751No Hit
ACTCGCCGAATCCCGGGGCCGAGGGNNNNNNNNNN7328390.4107762305818085No Hit
CTCCATCTAAGGCTAAATACCGNNNNNNNNNNNNN7287240.4084696609412134No Hit
AGGGCGCACCACCGGCCCGTCTCGCNNNNNNNNNN6431070.3604789992355417No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN6302950.3532975240872293No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN6010610.33691107041209933No Hit
CTAGCATCACCAGTATTAGAGGNNNNNNNNNNNNN5965640.3343903793613687No Hit
CCGCGACCGGCTCCGGGACGGCTGGGNNNNNNNNN5899290.3306712810465815No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNNN5837770.32722291739434783No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN5787540.32440738900924215No Hit
ATGTACACGGAGTCGACCCAACGCGACNNNNNNNN5512190.3089732711346884No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN5320020.29820161893947145No Hit
CACTCGCCGAATCCCGGGGCCGAGGNNNNNNNNNN5198600.2913956970497736No Hit
ATTGATCATCGACACTTCGAACGNNNNNNNNNNNN4895460.274403873942847No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN4643910.2603038109272523No Hit
AACGATGCCGACCGGCGATGCGGCGGCGNNNNNNN4549630.25501915999856906No Hit
AACGATGCCGACCGGCGATGCGGCGGCNNNNNNNN4416310.24754621067939164No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGNNNNNNN4322390.2422817387317683No Hit
CGCGACCGGCTCCGGGACGGCTGGGNNNNNNNNNN4270380.23936643649586659No Hit
TCGCTTCTGGCGCCAAGCGCCCGGNNNNNNNNNNN4269540.23931935220672684No Hit
ACGATGCCGACCGGCGATGCGGCGGCNNNNNNNNN4171920.23384748517598802No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNNN4125320.23123542818323622No Hit
CCTCTAGCATCACCAGTATTAGAGGCNNNNNNNNN3992560.22379386839015192No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN3936740.22066500527136643No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGANNNNNN3922990.21989428030032918No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGNNNNNN3627110.20330939997811032No Hit
GGGAGAGCGACGCTCAGACAGGCNNNNNNNNNNNN3622270.20303810478830517No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN3160210.17713838259794268No Hit
TGCTAAGACTTCACCAGTCAAAGCGNNNNNNNNNN3060050.17152414164528135No Hit
AGGGCGCACCACCGGCCCGTCTCGNNNNNNNNNNN3017470.16913741660769827No Hit
ATGCTCATAAGGAAAGGTTAAAAANNNNNNNNNNN2959400.16588243485728846No Hit
CGCTTCTGGCGCCAAGCGCCCGGNNNNNNNNNNNN2806260.15729852052531404No Hit
CGAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNN2660480.14912715425056391No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGNNNNN2499840.14012284447984186No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNNN2496490.1399350678505346No Hit
ATGCTCATAAGGAAAGGTTAAAANNNNNNNNNNNN2452660.1374782769064936No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATNNN2392720.13411847656002274No Hit
TTGATCATCGACACTTCGAACGCANNNNNNNNNNN2370090.1328500033895083No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCNNNNNN2337070.13099914240451552No Hit
ACGATGCCGACCGGCGATGCGGCGGCGNNNNNNNN2320800.130087164566059No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNN2307250.12932765014005504No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTNNNN2201450.12339727181745547No Hit
TGGTGACCTCCCGGGAGCGGGGGACCACCANNNNN2189940.12275210495079081No Hit
TCTAGCATCACCAGTATTAGAGNNNNNNNNNNNNN2095430.1174545618953193No Hit
GCGACCGGCTCCGGGACGGCTGGGNNNNNNNNNNN2054140.11514014487320084No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGAAGNNNN2048100.1148015864131961No Hit
ACCTCTAGCATCACCAGTATTAGAGGNNNNNNNNN2024110.11345688153645542No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN2012890.11282796996008901No Hit
GCCGCGACCGGCTCCGGGACGGCTGGNNNNNNNNN2002600.11225118741812729No Hit
CTAAACCATTCGTAGACGACCTGCTTNNNNNNNNN1956980.10969406209603852No Hit
TTCACTGACCCGGTGAGGCGGGGNNNNNNNNNNNN1919610.10759937175657211No Hit
ACTCGCCGAATCCCGGGGCCGAGGNNNNNNNNNNN1886880.10576476606187757No Hit
CCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN1877450.10523618886355891No Hit
CGCTTCTGGCGCCAAGCGCCCGGCNNNNNNNNNNN1800070.10089882899018696No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)