FastQCFastQC Report
Sun 24 Sept 2023
SRR20106156_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR20106156_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81323550
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length35-101
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNNN65789858.089889091167318No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNN55383036.810208113148036No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN31336543.853316782162117No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNN26758123.2903285702603977No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNN21104072.5950748583897285No Hit
CACTCGCCGAATCCCGGGGCCGAGGGANNNNNNNN17497772.1516239760807294No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNNN17410262.1408632554776568No Hit
CACTCGCCGAATCCCGGGGCCGAGGGNNNNNNNNN13045041.6040913118032845No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12653111.5558973999536418No Hit
CTCTAGCATCACCAGTATTAGAGGNNNNNNNNNNN12484151.5351211303490808No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN11886861.4616750006609402No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN9839231.2098869269725683No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNN9520741.1707236095817264No Hit
GCCGCGACCGGCTCCGGGACGGCTGGGNNNNNNNN8898871.0942549851795698No Hit
CCTCTAGCATCACCAGTATTAGAGGNNNNNNNNNN8315511.0225217664501856No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN7733100.9509053650510829No Hit
TCTAGCATCACCAGTATTAGAGGNNNNNNNNNNNN7264230.8932504791047612No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN7122230.8757893623679733No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN5900170.725518008006291No Hit
GAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNNN4691500.576893163173521No Hit
ACTCGCCGAATCCCGGGGCCGAGGGANNNNNNNNN4641650.5707633274740219No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN4350950.535017224407936No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCNNNNNNN3190610.39233530754621504No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGNNNNNNN2974880.36580793632348807No Hit
AACGATGCCGACCGGCGATGCGGCGGCGNNNNNNN2803930.34478696515339036No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN2795670.34377126920799694No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGANNNNNN2786890.3426916311449758No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNNN2779450.34177676700045684No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN2716140.3339918141792875No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGNNNNNN2464500.30304874787192637No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNNN2413120.2967307747878689No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN2299690.28278278555227854No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN2216760.2725852474467728No Hit
CTAAACCATTCGTAGACGACCTGCTTNNNNNNNNN2162940.2659672382723086No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN1949120.23967473136625247No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATNNNN1831620.22522627209461465No Hit
GGGAGAGCGACGCTCAGACAGGNNNNNNNNNNNNN1800820.22143893128128322No Hit
GGGAGAGCGACGCTCAGACAGGCNNNNNNNNNNNN1690300.20784877197318613No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNN1524580.1874709109476898No Hit
AACGATGCCGACCGGCGATGCGGCGGCNNNNNNNN1452850.1786505876833955No Hit
ATTGATCATCGACACTTCGAACGNNNNNNNNNNNN1433090.17622078721354392No Hit
TGCTAAGACTTCACCAGTCAAAGCGNNNNNNNNNN1415740.1740873338657744No Hit
TTGATCATCGACACTTCGAACGNNNNNNNNNNNNN1336310.16432017539814728No Hit
TTGATCATCGACACTTCGAACGCANNNNNNNNNNN1315080.16170961548038668No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN1256610.15451981621559807No Hit
CCTCTAGCATCACCAGTATTAGAGGCNNNNNNNNN1242930.15283764665954697No Hit
CTAGCATCACCAGTATTAGAGGNNNNNNNNNNNNN1240920.15259048578179385No Hit
TTCACTGACCCGGTGAGGCGGGGNNNNNNNNNNNN1222490.15032422957433608No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGNNNNN1208000.1485424578735188No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATNNN1155630.14210274883474713No Hit
GAGGGCGCACCACCGGCCCGTCTCGNNNNNNNNNN1146280.14095302037355723No Hit
CGAGGGCGCACCACCGGCCCGTCTCGNNNNNNNNN1138280.13996929548697762No Hit
ACTCGCCGAATCCCGGGGCCGAGGGNNNNNNNNNN1100520.13532611402232195No Hit
CACTCGCCGAATCCCGGGGCCGAGGNNNNNNNNNN1092720.13436698225790686No Hit
ATGCTCATAAGGAAAGGTTAAAANNNNNNNNNNNN1084360.13333898975143116No Hit
CCGCGACCGGCTCCGGGACGGCTGGGNNNNNNNNN1081800.1330241977877257No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNNN1067630.1312817750823716No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN1046580.12869334897455903No Hit
CGCGACCGGCTCCGGGACGGCTGGGNNNNNNNNNN1015670.12489248194403713No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGNNNNN1007090.1238374370031805No Hit
GCATGCTCATAAGGAAAGGTTAAAANNNNNNNNNN987520.12143099999938516No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN951850.1170448166613484No Hit
CCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN949960.11681241165689399No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGAAGNNNN889940.10943201569533056No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTNNNN862160.10601603102668293No Hit
CTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNNNN849510.10446051604977895No Hit
GAACCCCATTCGTGATGGGGATCGGGGATTNNNNN846810.10412850890055832No Hit
CGAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNN832490.10236764135358085No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)