FastQCFastQC Report
Sat 23 Sept 2023
SRR20106157_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR20106157_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196564651
Total Bases6.9 Gbp
Sequences flagged as poor quality0
Sequence length35-101
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN130516036.639852554160412No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNNN111535495.674239464348043No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNN103265145.253494942994608No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN83179944.231683549246095No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN70541033.588693574410793No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNN56794252.8893419905901596No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNN48179042.4510531143262377No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNNN33970611.7282156189924502No Hit
CACTCGCCGAATCCCGGGGCCGAGGGANNNNNNNN31930231.624413638849032No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN31762961.6159039704448181No Hit
CTCTAGCATCACCAGTATTAGAGGNNNNNNNNNNN24660161.2545572092715693No Hit
CACTCGCCGAATCCCGGGGCCGAGGGNNNNNNNNN24397061.2411723000998791No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN21370761.0872127766248267No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN20732381.0547359301139043No Hit
GCCGCGACCGGCTCCGGGACGGCTGGGNNNNNNNN19133410.9733901748183603No Hit
TCTAGCATCACCAGTATTAGAGGNNNNNNNNNNNN17016450.8656922754641169No Hit
CCTCTAGCATCACCAGTATTAGAGGNNNNNNNNNN16480750.8384391555732978No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN15840150.8058493691218163No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNN15818440.8047448979013017No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATNNNN14723300.7490309129895385No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTNNNN13159670.6694830394504656No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATNNN12565770.6392690616584973No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN11983030.6096228360001514No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN11260250.5728522367940917No Hit
TTTGAACCCCATTCGTGATGGGGATCGGGGATTNN10358510.5269772539112335No Hit
ACTCGCCGAATCCCGGGGCCGAGGGANNNNNNNNN8907060.45313640853970233No Hit
GAACCCCATTCGTGATGGGGATCGGGGATTNNNNN7983680.4061605156056264No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN7949310.4044119814808411No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGNNNNNNN7371710.37502724739658305No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGNNNNNN7190910.36582925584112275No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNNN6761050.3439606239272391No Hit
GAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNNN6548260.3331351780030886No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN6018460.3061822138101525No Hit
GAGGGCGCACCACCGGCCCGTCTCGNNNNNNNNNN5958170.30311502956856673No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCNNNNNNN5660630.2879780251027943No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN5375870.27349118840294434No Hit
AACGATGCCGACCGGCGATGCGGCGGCGNNNNNNN5197070.2643949445416816No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNNN5005230.25463530571425075No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGANNNNNN4697590.23898447539278056No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN4216700.21451975106144594No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN4160980.21168506030110165No Hit
CTAAACCATTCGTAGACGACCTGCTTNNNNNNNNN3988310.20290067312255447No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN3977240.20233749963517092No Hit
TGCTAAGACTTCACCAGTCAAAGCGNNNNNNNNNN3617570.184039703049151No Hit
AACCCCATTCGTGATGGGGATCGGGGATTNNNNNN3613890.18385248729182746No Hit
TTGATCATCGACACTTCGAACGNNNNNNNNNNNNN3332580.1695411653644683No Hit
ACCCCATTCGTGATGGGGATCGGGGATTNNNNNNN3293990.1675779436049262No Hit
CTAGCATCACCAGTATTAGAGGNNNNNNNNNNNNN3207280.16316667232299056No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGNNNNN3198300.1627098251760435No Hit
ATTGATCATCGACACTTCGAACGNNNNNNNNNNNN3164490.16098978040563358No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNNN3142740.159883274231235No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGN3103590.15789156311731758No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNN3032280.15426374908070323No Hit
ACTCGCCGAATCCCGGGGCCGAGGGNNNNNNNNNN2994130.15232291181388458No Hit
GGGAGAGCGACGCTCAGACAGGCNNNNNNNNNNNN2978910.15154861186104107No Hit
CCGCGACCGGCTCCGGGACGGCTGGGNNNNNNNNN2905010.14778903456044087No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNN2894010.14722942224235425No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGNNNNN2828710.1439073600268036No Hit
CCTCTAGCATCACCAGTATTAGAGGCNNNNNNNNN2810030.1429570365629983No Hit
GGGAGAGCGACGCTCAGACAGGNNNNNNNNNNNNN2758530.14033703343741089No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN2549720.12971406542471364No Hit
TTGATCATCGACACTTCGAACGCANNNNNNNNNNN2473210.1258217073831856No Hit
TTCACTGACCCGGTGAGGCGGGGNNNNNNNNNNNN2470740.1256960489808516No Hit
ATGCTCATAAGGAAAGGTTAAAANNNNNNNNNNNN2396350.121911543495173No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGAAGNNNN2367390.12043823688319219No Hit
TTTGAACCCCATTCGTGATGGGGATCGGGGATNNN2344470.11927220830768805No Hit
GTTGAACCCCATTCGTGATGGGGACCGGGGATTNN2324970.11828016828926173No Hit
AACGATGCCGACCGGCGATGCGGCGGCNNNNNNNN2315550.11780093664959118No Hit
CGCGACCGGCTCCGGGACGGCTGGGNNNNNNNNNN2309220.11747890519745587No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNNN2309140.11747483528968797No Hit
GCATGCTCATAAGGAAAGGTTAAAANNNNNNNNNN2228350.11336473718257715No Hit
CGAGGGCGCACCACCGGCCCGTCTCGNNNNNNNNN2175550.1106785980557613No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCNNNNN2124040.10805808619170292No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNNN2104750.10707672968116734No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNN2062840.10494460674925726No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGNNNNNNNN2030070.10327747077983009No Hit
CACTCGCCGAATCCCGGGGCCGAGGNNNNNNNNNN2026380.10308974628403557No Hit
CTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNNNN2009040.10220759377534265No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGG1988180.10114636532486199No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)