FastQCFastQC Report
Sat 23 Sept 2023
SRR20106158_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR20106158_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162014559
Total Bases5.7 Gbp
Sequences flagged as poor quality0
Sequence length35-101
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNN86386955.332048584596647No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN69784154.307276483714035No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNNN66007884.074194344472462No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN58504413.611058806140996No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN51610503.1855470470403837No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNN50287873.103910556581523No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNN50154863.095700800568176No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN36132932.2302273464201448No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNNN27954031.725402344859637No Hit
CACTCGCCGAATCCCGGGGCCGAGGGANNNNNNNN24703411.5247648206726903No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN22763721.4050416296229278No Hit
CTCTAGCATCACCAGTATTAGAGGNNNNNNNNNNN21084811.3014145228763052No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN20063111.238352289068046No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNN18809151.160954306581793No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN15181000.9370145555869458No Hit
CCTCTAGCATCACCAGTATTAGAGGNNNNNNNNNN13797580.851625933197769No Hit
CACTCGCCGAATCCCGGGGCCGAGGGNNNNNNNNN13363390.824826489821819No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN11431770.7056014021554692No Hit
TCTAGCATCACCAGTATTAGAGGNNNNNNNNNNNN11424410.7051471219941412No Hit
GCCGCGACCGGCTCCGGGACGGCTGGGNNNNNNNN9691840.5982079672234889No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATNNNN7126370.4398598523482078No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN7029020.43385113309477324No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATNNN6634420.40949529727140144No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN6493480.40079607907336273No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNNN6352280.3920808129348425No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCNNNNNNN5765700.3558754247511793No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN5353530.3304351184883329No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTNNNN5129820.3166271001607948No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGNNNNNN4890370.30184756420563413No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGANNNNNN4883860.3014457484651117No Hit
ACTCGCCGAATCCCGGGGCCGAGGGANNNNNNNNN4827390.29796025923818364No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNNN4684570.2891450020858928No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN4466940.2757122586742343No Hit
AACGATGCCGACCGGCGATGCGGCGGCGNNNNNNN4074750.25150517491455815No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGNNNNNNN3934190.24282941139876202No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN3816850.23558685241367722No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN3685730.22749375258306262No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN3667080.22634262146774106No Hit
GAACCCCATTCGTGATGGGGATCGGGGATTNNNNN3591940.2217047666685313No Hit
TTTGAACCCCATTCGTGATGGGGATCGGGGATTNN3113250.19215865655629133No Hit
CTAAACCATTCGTAGACGACCTGCTTNNNNNNNNN3068350.1893873006807987No Hit
GGGAGAGCGACGCTCAGACAGGCNNNNNNNNNNNN3037430.18747883022043715No Hit
CGAGGGCGCACCACCGGCCCGTCTCGNNNNNNNNN2793850.1724443789030096No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNN2752520.1698933735949002No Hit
GGGAGAGCGACGCTCAGACAGGNNNNNNNNNNNNN2688460.16593940795160267No Hit
TGCTAAGACTTCACCAGTCAAAGCGNNNNNNNNNN2549510.15736301822109705No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNNN2453900.15146169672319387No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGNNNNN2217980.1369000424214962No Hit
GAGGGCGCACCACCGGCCCGTCTCGNNNNNNNNNN2208390.13630812030911368No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN2194790.13546868957622504No Hit
TTGATCATCGACACTTCGAACGCANNNNNNNNNNN2186840.13497799293457324No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGAAGNNNN2156050.13307754644445255No Hit
CCTCTAGCATCACCAGTATTAGAGGCNNNNNNNNN2074080.1280181245933583No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCNNNNN2059830.12713857400926543No Hit
GAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNNN2050600.12656887212216528No Hit
TTGATCATCGACACTTCGAACGNNNNNNNNNNNNN1978470.12211680309545514No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNNN1926650.11891832511175739No Hit
ACCCCATTCGTGATGGGGATCGGGGATTNNNNNNN1916400.1182856659196906No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGNNNNN1909220.1178424958710038No Hit
GCATGCTCATAAGGAAAGGTTAAAANNNNNNNNNN1858490.11471129579163314No Hit
ATGCTCATAAGGAAAGGTTAAAANNNNNNNNNNNN1836310.11334228302284859No Hit
AACCCCATTCGTGATGGGGATCGGGGATTNNNNNN1809660.11169736912347487No Hit
ATGCTCATAAGGAAAGGTTAAAAANNNNNNNNNNN1687700.10416965058059999No Hit
CTAGCATCACCAGTATTAGAGGNNNNNNNNNNNNN1654410.10211489697046301No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)