FastQCFastQC Report
Sat 23 Sept 2023
SRR20106159_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR20106159_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182090437
Total Bases6.4 Gbp
Sequences flagged as poor quality0
Sequence length35-101
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNNN148934178.179131889282028No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNN101125895.553607958006054No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN75744024.159692362098071No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN69152463.797698612805241No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNN57217543.142259469672205No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNN48209742.647571217592278No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN44181902.426371243208121No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNNN29441731.616874037157701No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN27638531.5178463216055658No Hit
CACTCGCCGAATCCCGGGGCCGAGGGANNNNNNNN26823981.4731130553550158No Hit
GCCGCGACCGGCTCCGGGACGGCTGGGNNNNNNNN23787641.3063640459053871No Hit
CACTCGCCGAATCCCGGGGCCGAGGGNNNNNNNNN22449911.2328989028676998No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN20789511.1417134442925194No Hit
CTCTAGCATCACCAGTATTAGAGGNNNNNNNNNNN20524221.1271443101649539No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNN18534501.0178733329087457No Hit
CCTCTAGCATCACCAGTATTAGAGGNNNNNNNNNN16823590.9239139779756803No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN15773240.8662311025152848No Hit
GAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNNN15541750.8535181888766624No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN10709800.5881582897184217No Hit
TCTAGCATCACCAGTATTAGAGGNNNNNNNNNNNN9357760.5139072734500604No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN8603740.47249817957216506No Hit
ACTCGCCGAATCCCGGGGCCGAGGGANNNNNNNNN7952390.4367274927238491No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN7552060.41474226348306253No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNNN7204740.3956682250150237No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATNNN7153380.3928476485560854No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCNNNNNNN6843120.3758088624939705No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN6674070.36652501416095784No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATNNNN6605640.36276699143733726No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGNNNNNNN6491050.35647396463769265No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTNNNN6476460.3556727144325542No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGANNNNNN6203110.34066094311147155No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5964070.32753340033996403No Hit
AACGATGCCGACCGGCGATGCGGCGGCGNNNNNNN5964000.3275295560963479No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN5300620.2910982085237128No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN5238440.28768342183724893No Hit
TTTGAACCCCATTCGTGATGGGGATCGGGGATTNN5088230.27943422421464126No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGNNNNNN4915460.2699460817923129No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN4788640.2629814107151602No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNNN4576380.25132456571566136No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN4204980.23092810744366546No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNN4043140.2220402162031167No Hit
GAACCCCATTCGTGATGGGGATCGGGGATTNNNNN3910950.214780636722839No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGNNNNN3781050.20764681892657547No Hit
CTAAACCATTCGTAGACGACCTGCTTNNNNNNNNN3270010.17958164381801114No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNNN3126480.17169929687191643No Hit
CCGCGACCGGCTCCGGGACGGCTGGGNNNNNNNNN3077370.16900228538635448No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGAAGNNNN2978780.16358794284183084No Hit
AACGATGCCGACCGGCGATGCGGCGGCNNNNNNNN2901170.1593257750268346No Hit
CCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN2776490.15247862796880432No Hit
GAGGGCGCACCACCGGCCCGTCTCGNNNNNNNNNN2665320.1463734199286918No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNN2623230.14406193116006416No Hit
CGAGGGCGCACCACCGGCCCGTCTCGNNNNNNNNN2502080.13740864381581994No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNNN2475850.13596815081508098No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNN2445030.13427558526865419No Hit
CCTCTAGCATCACCAGTATTAGAGGCNNNNNNNNN2433590.13364732602624266No Hit
GCATGCTCATAAGGAAAGGTTAAAANNNNNNNNNN2378370.13061476699075636No Hit
CGCGACCGGCTCCGGGACGGCTGGGNNNNNNNNNN2270310.12468035320273299No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGNNNNN2234420.12270935458296471No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGN2195270.12055932404621557No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN2195180.1205543814472805No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGNNNN2143930.1177398459425961No Hit
TGCTAAGACTTCACCAGTCAAAGCGNNNNNNNNNN2143340.11770744446068851No Hit
ATGCTCATAAGGAAAGGTTAAAANNNNNNNNNNNN2101350.11540144746865535No Hit
ACTCGCCGAATCCCGGGGCCGAGGGNNNNNNNNNN2011460.11046488948785378No Hit
ACCCCATTCGTGATGGGGATCGGGGATTNNNNNNN1964540.10788814790971146No Hit
TGGTGACCTCCCGGGAGCGGGGGACCACCANNNNN1932110.10610716476011314No Hit
AACCCCATTCGTGATGGGGATCGGGGATTNNNNNN1898370.10425423933712674No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGNNNNNNNN1866970.10252982148645182No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)