FastQCFastQC Report
Sat 23 Sept 2023
SRR20106160_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR20106160_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184517112
Total Bases6.4 Gbp
Sequences flagged as poor quality0
Sequence length35-101
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNNN93815335.084370169418216No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTNNN83219264.510110693690025No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTNN70299723.809929563606003No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN57582523.1207143541245106No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNN57177833.098781970964297No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNN45570492.4697161962951166No Hit
ATGTACACGGAGTCGACCCAACGCGACNNNNNNNN44805062.4282333228801023No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNN44613742.4178646368581793No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN40179282.177536791276031No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNN38227592.0717639456659174No Hit
GAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNNN32587381.7660898572919352No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNN26310051.4258867220943714No Hit
GAGGGCGCACCACCGGCCCGTCTCGNNNNNNNNNN23321511.2639212562572517No Hit
CACTCGCCGAATCCCGGGGCCGAGGGNNNNNNNNN22385931.213217015883058No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNN19492671.0564152987610167No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATNNNN18602911.0081942969061861No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN18422650.9984250132854887No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNNNN18301140.9918397161993301No Hit
GCCGCGACCGGCTCCGGGACGGCTGGGNNNNNNNN15487220.839337871275592No Hit
CTCTAGCATCACCAGTATTAGAGGNNNNNNNNNNN13697350.742334943980697No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNN13501350.7317126229463206No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNN12997060.7043823664441485No Hit
CACTCGCCGAATCCCGGGGCCGAGGGANNNNNNNN12923590.7004006219217218No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNN11158960.6047655894375802No Hit
CCTCTAGCATCACCAGTATTAGAGGNNNNNNNNNN10561350.5723778074306735No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATNNN9153390.4960726894533229No Hit
TTTGAACCCCATTCGTGATGGGGATCGGGGATTNN8992260.48734016604378677No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNN8566360.46425829600021057No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNN7743100.4196412959249004No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCNNNNNNN7666220.41547474469468176No Hit
GGGAGAGCGACGCTCAGACAGGNNNNNNNNNNNNN7644290.4142862370401722No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTNNNN7286670.39490483679367366No Hit
TCTAGCATCACCAGTATTAGAGGNNNNNNNNNNNN6777040.36728517623883034No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGNNNNNNNN6720220.3642057870491708No Hit
ACTCGCCGAATCCCGGGGCCGAGGGANNNNNNNNN6423560.3481281454264253No Hit
ATGTACACGGAGTCGACCCAACGCGNNNNNNNNNN6082750.329657771795171No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNN5965970.3233288194972399No Hit
CGAGGGCGCACCACCGGCCCGTCTCGNNNNNNNNN5836750.3163256749867188No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNNN5458110.2958050850047989No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGNNNNNN5025720.2723714860657477No Hit
ACTCGCCGAATCCCGGGGCCGAGGGNNNNNNNNNN4166130.22578556291299423No Hit
GAACCCCATTCGTGATGGGGATCGGGGATTNNNNN4069950.22057303823398233No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGNNNNNNN3946680.21389235704057627No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGANNNNNN3911150.21196679037551813No Hit
AACGATGCCGACCGGCGATGCGGCGGCGNNNNNNN3859760.2091816828349232No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNNN3845110.20838771853311902No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGNNNN3728580.20207231511405835No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGNNNNNN3714720.2013211652694846No Hit
CCGCGACCGGCTCCGGGACGGCTGGGNNNNNNNNN3704860.20078679748683687No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNN3594150.19478681196787861No Hit
AACGATGCCGACCGGCGATGCGGCGGCNNNNNNNN3481010.18865513134630027No Hit
TGTACACGGAGTCGACCCAACGCGACNNNNNNNNN3281720.17785450706598963No Hit
TTTGAACCCCATTCGTGATGGGGATCGGGGATNNN3277820.17764314455561173No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGAAGNNNN3144380.17041129496975868No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGNN3139990.17017337665679483No Hit
AGGGCGCACCACCGGCCCGTCTCGCNNNNNNNNNN3045310.16504214525100522No Hit
CACTCGCCGAATCCCGGGGCCGAGGNNNNNNNNNN3041110.16481452408598288No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNN3032570.16435169438377076No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGNNNNN2849030.15440464947229393No Hit
CGCGACCGGCTCCGGGACGGCTGGGNNNNNNNNNN2747200.1488859201308115No Hit
TTGATCATCGACACTTCGAACGNNNNNNNNNNNNN2671650.1447914489361832No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGNNNN2571540.13936593588132898No Hit
GGGAGAGCGACGCTCAGACAGGCNNNNNNNNNNNN2547920.13808583780565564No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNNN2451420.13285597056168968No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCNNNNNN2403200.13024266280517116No Hit
CTAGCATCACCAGTATTAGAGGNNNNNNNNNNNNN2359480.12787323486831942No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGNNNN2129440.11540609848695224No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTNNNNN2108800.11428750304741384No Hit
TCGCTTCTGGCGCCAAGCGCCCGGNNNNNNNNNNN2022310.10960013291341782No Hit
ACGATGCCGACCGGCGATGCGGCGGCNNNNNNNNN2020780.10951721377473109No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGNNNNNN1967280.10661775369647017No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGNNN1949520.1056552413415185No Hit
GGGAGAGCGACGCTCAGACAGGCGTAGCCCCNNNN1936880.10497021002583218No Hit
AACCCCATTCGTGATGGGGATCGGGGATTNNNNNN1917350.10391177160847824No Hit
ACCCCATTCGTGATGGGGATCGGGGATTNNNNNNN1898210.1028744694421621No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGNNNNN1878420.10180194019078295No Hit
CCTCTAGCATCACCAGTATTAGAGGCNNNNNNNNN1878390.10180031432531851No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTTNNNNN1867010.1011835693591389No Hit
CGCTTCTGGCGCCAAGCGCCCGGNNNNNNNNNNNN1866040.10113099970912183No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)